Transgenic Mouse
Humana Press Inc. (Verlag)
978-1-4939-9839-5 (ISBN)
Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field.
The History of Transgenesis.- Pronuclear Microinjection of One-Cell Embryos.- Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection.- In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos.- Targeted Mutations in the Mouse via Embryonic Stem Cells.- Blastocyst Microinjection with Embryonic Stem Cells.- Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos.- Embryo Transfer Surgery.- Non-Surgical Embryo Transfer Protocol for use with the NSET™ Device.- Nuclear Transfer and Cloning.- Sperm-Mediated Genetic Modifications.- Genotyping Genetically Modified (GM) Mice.- Nomenclature: Naming Your Gene-Modified Mouse.- Breeding Strategies for Genetically Modified Mice.- Strategies for Behaviorally Phenotyping the Transgenic Mouse.- Cryobanking and Recovery of Genetically Modified Mice.- Simple Transportation of Genetically EngineeredMice via Cold Storage Techniques.- Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells using Sendai Virus.
Erscheinungsdatum | 13.09.2020 |
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Reihe/Serie | Methods in Molecular Biology ; 2066 |
Zusatzinfo | 27 Illustrations, color; 18 Illustrations, black and white; X, 236 p. 45 illus., 27 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Zoologie | |
Schlagworte | Embryonic Stem Cells • Enzymatic modifier systems • nuclear transfer • pronuclear injection • Spermatogonial |
ISBN-10 | 1-4939-9839-0 / 1493998390 |
ISBN-13 | 978-1-4939-9839-5 / 9781493998395 |
Zustand | Neuware |
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