Imaging Gene Expression
Humana Press Inc. (Verlag)
978-1-4939-9676-6 (ISBN)
Imaging Single mRNA Molecules in Mammalian Cells using an Optimized MS2-MCP System.- Live-Cell Imaging of Long Non-Coding RNAs using Molecular Beacons.- Real-Time Observation of Localization and EXpression (ROLEX) System for Live Imaging of the Transcriptional Activity and Nuclear Position of a Specific Endogenous Gene.- Visualization of Single mRNAs in Live Neurons.- Lightening Up Gene Activation in Living Drosophila Embryos.- Quantification of mRNA Turnover in Living Cells: A Pipeline for TREAT Data Analysis.- Imaging rRNA Methylation in Bacteria by MR-FISH.- Detection of mRNA Transfer between Mammalian Cells in Co-Culture by Single Molecule Fluorescent In Situ Hybridization (smFISH).- Live-Cell Imaging of mRNP-NPC Interactions in Budding Yeast.- Detection of mRNAs Anchored to the Nuclear Envelope during Export Inhibition in Living Cells.- Programmable Chromosome Painting with Oligopaints.- Imaging Chromosome Territory and Gene Loci Positions in Cells Grown on Soft Matrices.- Targeted Manipulation/Repositioning of Subcellular Structures and Molecules.- Visualization of Endogenous Transcription Factors in Single Cells using an Antibody Electroporation-Based Imaging Approach.- Monitoring Transcription Factor Oligomerization in Single Living Cells by Numbers and Brightness Analysis.- Visualizing Transcription Factor Binding on Mitotic Chromosomes using Single Molecule Live-Cell Imaging.- Light-Induced Transcription Activation for Time-Lapse Microscopy Experiments in Living Cells.
Erscheinungsdatum | 14.08.2020 |
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Reihe/Serie | Methods in Molecular Biology ; 2038 |
Zusatzinfo | 46 Illustrations, color; 1 Illustrations, black and white; XIII, 272 p. 47 illus., 46 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik | |
Schlagworte | CRISPR • Drosophila • Endoplasmic Reticulum. • fish • mRNAs • RNA Pol II |
ISBN-10 | 1-4939-9676-2 / 1493996762 |
ISBN-13 | 978-1-4939-9676-6 / 9781493996766 |
Zustand | Neuware |
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