Neural Crest Cells

Methods and Protocols

Quenten Schwarz, Sophie Wiszniak (Herausgeber)

Buch | Softcover

230 Seiten

2020 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9414-4 (ISBN)

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This detailed book provides a wide range of techniques, from those that have been used extensively since the very first investigations into neural crest cells to those that are currently cutting-edge, in order to explore the development of neural crest cells in human, mice, rat, chick, quail, medaka, and shark. With a bit of imagination and adjustment, many of these methodologies can be adaptable to any species desirable for study. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Neural Crest Cells: Methods and Protocols serves as an ideal reference guide for aspiring and experienced developmental biologists alike.

The neural crest: a remarkable model system for studying development and disease.- Addressing interdisciplinary difficulties in developmental biology / mathematical collaborations: a neural crest example.- Generation of neural crest progenitors from human pluripotent stem cells.- Fully defined and xeno-free induction of hPSCs into neural crest using top-down inhibition of BMP signaling.- Deciphering the neural crest contribution to cephalic development with avian embryos.- In ovo electroporation of plasmid DNA and morpholinos into specific tissues during early embryogenesis.- Ex vivo culture and manipulation of mouse neural crest cells from primary embryonic tissue explants.- Technologies for live imaging of enteric neural crest derived cells.- Mouse embryo culture for the study of neural crest cells.- Laser capture microdissection of murine embryonic neural crest cells.- In vivo and in vitro quantitative analysis of neural crest cell migration.- Proteomics in neural crest cell research.- Neural crest methodologies in zebrafish and medaka.- Fluorescence-activated cell sorting and nanostring profiling of single neural crest cells and pigment cells.- Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity.- Avenues for investigating the neural crest and its derivatives in non-model (unconventional) vertebrates: a craniofacial skeleton perspective.

Erscheinungsdatum	07.07.2020
Reihe/Serie	Methods in Molecular Biology ; 1976
Zusatzinfo	36 Illustrations, color; X, 230 p. 36 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Animal Models • Cell Migration • Embryology • Epithelial-to-mesenchymal transition • model systems
ISBN-10	1-4939-9414-X / 149399414X
ISBN-13	978-1-4939-9414-4 / 9781493994144
Zustand	Neuware