The Integrin Interactome

Methods and Protocols

Buch | Hardcover

315 Seiten

2020 | 1st ed. 2021
Springer-Verlag New York Inc.
978-1-0716-0961-3 (ISBN)

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This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.

Measurement of Integrin Activation and Conformational Changes on the Cell Surface by Soluble Ligand and Antibody Binding Assays.- Quantification of Integrin Activation and Ligation in Adherent Cells.- Multi-parametric Analysis of Focal Adhesions in Bi-Dimensional Substrates.- Focal Adhesion Isolation Assay using ECM Coated Magnetic Beads.- Functional Integrin Regulation through Interactions with Tetraspanin CD9.- Proximity-dependent Biotinylation (BioID) of Integrin Interaction Partners.- Analyzing the Integrin Adhesome by in situ Proximity Ligation Assay.- Single-Protein Tracking to Study Protein Interactions during integrin-based Migration.- Biochemical Characterization of the Integrin Interactome.- Network Analysis of Integrin Adhesion Complexes.- Surface Patterning for the Control of Receptor Clustering and Molecular Forces of Integrin-mediated Adhesions.- Dynamics and Physics of Integrin Activation in Tumour Cells by Nano-Sized Extracellular Ligands andElectromagnetic Fields.- Genetic Instruction of Megakaryocytes and Platelets Derived from Human Induced Pluripotent Stem Cells for Studies of Integrin Regulation.- Quantitative Analysis of Integrin Trafficking.- Methods to Study Integrin Functions on Exosomes.- Functional Bioinformatics Analyses of the Matrisome and Integrin Adhesome.- Quantifying Polarized Extracellular Matrix Secretion in Cultured Endothelial Cells.

Erscheinungsdatum	16.01.2021
Reihe/Serie	Methods in Molecular Biology ; 2217
Zusatzinfo	43 Illustrations, color; 11 Illustrations, black and white; XIX, 315 p. 54 illus., 43 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	adhesome • affinity techniques • epigenetic • FRET • Translational Medicine
ISBN-10	1-0716-0961-0 / 1071609610
ISBN-13	978-1-0716-0961-3 / 9781071609613
Zustand	Neuware