Nicht aus der Schweiz? Besuchen Sie lehmanns.de
Yeast Protocols -

Yeast Protocols

Wei Xiao (Herausgeber)

Buch | Hardcover
260 Seiten
2020 | 4th ed. 2021
Springer-Verlag New York Inc.
978-1-0716-0867-8 (ISBN)
CHF 299,55 inkl. MwSt
  • Versand in 10-20 Tagen
  • Versandkostenfrei
  • Auch auf Rechnung
  • Artikel merken
This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community.

 Use of Yeast Plasmids - Transformation and Inheritance Assays.- Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae.- CRISPR Nickase-Mediated Base Editing in Yeast.- Genomic Promoter Shuffling by Using Recyclable Cassettes.- Study Essential Gene Functions by Plasmid Shuffling.- Scarless Genomic Protein Labeling in Saccharomyces cerevisiae.- High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae.- Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections.- Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe.- Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map.- Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly.- Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE.- Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering.- A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9.- YeastNucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA.- Assays for Autophagy III: Observing Dynamic Protein Trafficking.- Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification.- High-Throughput Rapid Yeast Chronological Lifespan Assay.- Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast.- Monitoring 5’-end Resection at Site-specific Double-strand Breaks by Southern Blot Analysis. 

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 2196
Zusatzinfo 44 Illustrations, color; 11 Illustrations, black and white; XI, 260 p. 55 illus., 44 illus. in color.
Verlagsort New York, NY
Sprache englisch
Maße 178 x 254 mm
Themenwelt Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
Schlagworte CRISPR-Cas9 • fluorescent cellular proteins • Homologous recombination • nuclear extracts • Nucleosome
ISBN-10 1-0716-0867-3 / 1071608673
ISBN-13 978-1-0716-0867-8 / 9781071608678
Zustand Neuware
Haben Sie eine Frage zum Produkt?
Mehr entdecken
aus dem Bereich
50 Meilensteine der Genetik

von Jonathan Weitzman; Matthew Weitzman

Buch | Hardcover (2022)
Librero b.v. (Verlag)
CHF 13,90