Yeast Protocols
Springer-Verlag New York Inc.
978-1-0716-0867-8 (ISBN)
Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community.
Use of Yeast Plasmids - Transformation and Inheritance Assays.- Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae.- CRISPR Nickase-Mediated Base Editing in Yeast.- Genomic Promoter Shuffling by Using Recyclable Cassettes.- Study Essential Gene Functions by Plasmid Shuffling.- Scarless Genomic Protein Labeling in Saccharomyces cerevisiae.- High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae.- Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections.- Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe.- Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map.- Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly.- Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE.- Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering.- A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9.- YeastNucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA.- Assays for Autophagy III: Observing Dynamic Protein Trafficking.- Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification.- High-Throughput Rapid Yeast Chronological Lifespan Assay.- Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast.- Monitoring 5’-end Resection at Site-specific Double-strand Breaks by Southern Blot Analysis.
Erscheinungsdatum | 11.09.2020 |
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Reihe/Serie | Methods in Molecular Biology ; 2196 |
Zusatzinfo | 44 Illustrations, color; 11 Illustrations, black and white; XI, 260 p. 55 illus., 44 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | CRISPR-Cas9 • fluorescent cellular proteins • Homologous recombination • nuclear extracts • Nucleosome |
ISBN-10 | 1-0716-0867-3 / 1071608673 |
ISBN-13 | 978-1-0716-0867-8 / 9781071608678 |
Zustand | Neuware |
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