Virus-Induced Gene Silencing in Plants

Methods and Protocols

Vincent Courdavault, Sébastien Besseau (Herausgeber)

Buch | Hardcover

261 Seiten

2020 | 1st ed. 2020
Springer-Verlag New York Inc.
978-1-0716-0750-3 (ISBN)

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This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field.

irus-induced Gene Silencing in Lilies using Cucumber Mosaic Virus Vectors.- Virus-Induced Gene Silencing in Poaceae Using a Foxtail Mosaic Virus Vector.- Virus-Induced Gene Silencing (VIGS) in Chili Pepper (Capsicum spp.).- Virus-Induced Gene Silencing in Diploid and Tetraploid Potato Species.- Virus Induced Gene Silencing (VIGS) in Cassava using Geminivirus Agroclones.- Virus Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum).- Virus-Induced Gene Silencing to Investigate Alkaloid Biosynthesis in Opium Poppy.- A Biolistic-mediated Virus-Induced Gene Silencing in Apocynaceae to Map Biosynthetic Pathways of Alkaloids.- Virus Induced Gene Silencing in Nepeta.- Virus Induced Gene Silencing in Sweet basil (Ocimum basilicum).- Virus-Induced Gene Silencing for Gene Function Studies in Withania somnifera, an Important Indian Medicinal Plant.- A Prunus necrotic ringspot virus(PNRSV)-based Viral Vector for Characterization of Gene Functions in Prunus Fruit Trees.- Virus-Induced Gene Silencing in Olive Tree (Oleaceae).- ALSV-based Virus-Induced Gene Silencing in Apple Tree (Malus x domestica L.).- Virus-Induced Gene Silencing for Functional Analysis of Flower Traits in Petunia.- Virus-induced Gene Silencing in Rose Flowers.- Virus-induced Gene Silencing (VIGS) in Flax (Linum usitatissimum L.) Seedcoat: Description of an Effective Procedure using the transparent testa 2 Gene as a Selectable Marker.- Virus-Based microRNA Silencing in Plants.

Erscheinungsdatum	29.06.2020
Reihe/Serie	Methods in Molecular Biology ; 2172
Zusatzinfo	48 Illustrations, color; 10 Illustrations, black and white; XII, 261 p. 58 illus., 48 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	Apocynaceae • benzylisoquinoline alkaloid biosynthesis • Brachypodium • geminivirus • Gossypium ssp
ISBN-10	1-0716-0750-2 / 1071607502
ISBN-13	978-1-0716-0750-3 / 9781071607503
Zustand	Neuware