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Key to Successful "in Situ" Pcr

Gerard J. Nuovo (Herausgeber)

VHS Video
1996
Lippincott Williams and Wilkins (Hersteller)
978-0-397-58751-3 (ISBN)
CHF 154,65 inkl. MwSt
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This practical, hands-on video training programme is designed for pathologists and laboratory scientists who are beginning to use "in situ" polymerase chain reaction (PCR). It presents a step-by-step demonstration that shows viewers how to succesfully use the technique in their own laboratories.
This practical, hands-on video training programme is designed for pathologists and laboratory scientists who are beginning to use "in situ" polymerase chain reaction (PCR) - a diagnostic technique for infectious diseases and cancer. This video presents a step-by-step demonstration that shows viewers how to succesfully use the technique in their own laboratories. Part 1 of the video reviews key preparatory steps and includes information on the protease, fixative, hot start manoeuvre and reagents. Part 2 demostrates the actual techniques of "in situ" PCR and "in situ" hybridization. The third part of the video goes on to explain the basics of molecular biology and histology so that viewers can interpret "in situ" PCR findings.

The basics of molecular biology and histological analysis; the nonspecific pathways of PCR; "in situ" hybridization; PCR "in situ" hybridization; a starter's guide to "in situ" PCR - the start-up protocol; reverse transcriptase "in situ: PCR; applications of PCR "in situ" hybridization - human papilomavirus; applications of PCR "in situ" hybridization - Human Immunodeficiency Virus-1; instrumentation for "in situ" PCR, John G. Atwood. Appendix - glossary of terms and reagents for molecular biological analysis.