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MERS Coronavirus -

MERS Coronavirus

Methods and Protocols

Rahul Vijay (Herausgeber)

Buch | Hardcover
XI, 224 Seiten
2019
Springer-Verlag New York Inc.
978-1-0716-0210-2 (ISBN)
CHF 299,55 inkl. MwSt
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This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-viral factors, and mouse models for MERS -coronavirus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and cutting-edge, MERS Coronavirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.

PART I: Evolution and Entry of MERS-Coronavirus



1. Studying Evolutionary Adaptation of MERS-CoV



Michael Letko and Vincent Munster







2. Evaluating MERS-CoV Entry Pathways



Enya Qing, Michael P Hantak, Gautami G Galpalli, and Tom Gallagher







3. Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing



Gary R. Whittaker and Jean K. Millet







4. Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein



Haixia Zhou, Shuyuan Zhang, and Xinquan Wang







PART II: Genetic Alteration and Structural Determination of MERS-Coronavirus Proteins



5. Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV



Anthony R. Fehr







6. Deducing the Crystal Structure of MERS-CoV Helicase



Sheng Cui and Wei Hao







PART III: Quantitation of Virus and Anti-Viral Factors



7. Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans



Joshua Fung, Susanna K. P. Lau, and Patrick C. Y. Woo







8. Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR



Abdullah Algaissi, Anurodh S. Agrawal, Anwar M. Hashem, and Chien-Te K. Tseng







9. Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay Abdullah Algaissi and Anwar M Hashem







10. Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera Abdulrahman Almasaud, Naif Khalaf Alharbi, and Anwar M Hashem







11. Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA



Sawsan S Al-amri and Anwar M Hashem







PART IV: Mouse Models for MERS -Coronavirus



12. Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced



Acute Respiratory Distress Syndrome



Sarah R. Leist and Adam S. Cockrell







13. Development of a Mouse Adapted MERS Coronavirus



Kun Li and Paul B. McCray, Jr.







14. Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection



Carrie D. Nicora, Amy C. Sims, Kent J. Bloodsworth, Young-Mo Kim, Ronald J. Moore, Jennifer E. Kyle, Ernesto S. Nakayasu, and Thomas O. Metz







15. Evaluation of Activation and Inflammatory Activity of Myeloid Cells During



Pathogenic Human Coronavirus Infection



Rudragouda Channappanavar and Stanley Perlman



16. Histopathologic Evaluation and Scoring of Viral Lung Infection David K. Meyerholz and Amanda P. Beck

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 2099
Zusatzinfo 26 Tables, color; 27 Illustrations, color; 13 Illustrations, black and white
Verlagsort New York, NY
Sprache englisch
Maße 178 x 254 mm
Gewicht 680 g
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete Mikrobiologie / Infektologie / Reisemedizin
Studium Querschnittsbereiche Infektiologie / Immunologie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
ISBN-10 1-0716-0210-1 / 1071602101
ISBN-13 978-1-0716-0210-2 / 9781071602102
Zustand Neuware
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