The LINC Complex
Humana Press Inc. (Verlag)
978-1-4939-9367-3 (ISBN)
Authoritative and cutting-edge, The LINC Complex: Methods and Protocols aims to ensure successful results in the further study of this vital field.
EM Tomography of LINC Complexes.- Recombinant Purification of the Periplasmic Portion of the LINC Complex.- Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis.- Analysis of Nesprin-2 Interaction with its Binding Partners and Actin.- Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID.- Using Nesprin Tension Sensors to Measure Force on the LINC Complex.- Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei.- Direct Force Probe for Nuclear Mechanics.- Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning.- Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments.- Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy.- Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super Resolution Imaging.- Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans.- High-resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development.- Computational Methods for Studying the Plant Nucleus.- Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System.- Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components.- Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice.- An in vitro System to Measure the Positioning, Stiffness and Rupture of the Nucleus in Skeletal Muscle.- Functional Analysis of LINC Complexes in Skin.- Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer.- Next-generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins
Erscheinungsdatum | 07.10.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 1840 |
Zusatzinfo | 41 Illustrations, color; 20 Illustrations, black and white; XIV, 338 p. 61 illus., 41 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | Cytoskeleton • KASH domain • Nucleoskeleton • SUN domain • tissue-selective expression |
ISBN-10 | 1-4939-9367-4 / 1493993674 |
ISBN-13 | 978-1-4939-9367-3 / 9781493993673 |
Zustand | Neuware |
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