Genotype Phenotype Coupling
Humana Press Inc. (Verlag)
978-1-4939-9852-4 (ISBN)
Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field.
Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin.- Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins.- cDNA Display: A Stable and Simple Genotype–phenotype Coupling Using a Cell-free Translation System.- cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution.- Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display.- Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library.- In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties.- Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens.- Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning.- Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display.- Selection and Characterization of Anti-idiotypic Shark Antibody Domains.-Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression.- Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors.- Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting.- Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires.- Rapid Generation of Chicken Immune Libraries for Yeast Surface Display.- Ligand Engineering via Yeast Surface Display and Adherent Cell Panning.- Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping.- Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies.- Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors.- Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets.- Engineering Antibodies on the Surface of CHO Cells.- Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies.
Erscheinungsdatum | 08.11.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 2070 |
Zusatzinfo | 61 Illustrations, color; 25 Illustrations, black and white; XII, 445 p. 86 illus., 61 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Biochemie | |
Schlagworte | Aptamers • genotype phenotype coupling • phage-assisted Continuous Evolution (PACE) • Phage Display • SELEX |
ISBN-10 | 1-4939-9852-8 / 1493998528 |
ISBN-13 | 978-1-4939-9852-4 / 9781493998524 |
Zustand | Neuware |
Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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