Planarian Regeneration

Methods and Protocols

Buch | Softcover

573 Seiten

2019 | Softcover reprint of the original 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-9289-8 (ISBN)

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This volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental protocols for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  

Comprehensive and cutting-edge, Planarian Regeneration: Methods and Protocols is a valuable resource for both newcomers to the field and experts within established planarian laboratories.

Jochen Rink, PhD Group Leader Max Planck Institute of Molecular Cell Biology and Genetics Pfotenhauerstrasse 108, 01307 Dresden, Germany Tel: +49-(0)351-210-2435 Email: rink@mpi-cbg.de

Planarian Diversity and Phylogeny.- Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal Scale.- The Ecology of Freshwater Planarians.- Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in Biology.- Culturing Planarians in the Laboratory.- Total RNA Isolation from Planarian Tissues.- Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians.- Planarian High Molecular Weight DNA Isolation by Spooling.- Basic Histological Techniques for Planarians.- Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians.- Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections.- Whole-Mount In Situ Hybridization of Planarians.- RNA In Situ Hybridization on Planarian Paraffin Sections.- General Principles of Planarian Embryogenesis and Its Analysis by In Situ Hybridization and Immunohistochemistry Methods.- Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians.- Detection of Apoptotic Cells in Planarians by Whole-Mount TUNEL.- Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria.- RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNA.- A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural Cells.- Clonal Analysis of Planarian Stem Cells by Subtotal Irradiation and Single-Cell Transplantation.- Tissue Transplantations in Planarians.- Live Imaging Planaria.- Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave Techniques.- Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILAC.- Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.

Erscheinungsdatum	01.08.2019
Reihe/Serie	Methods in Molecular Biology ; 1774
Zusatzinfo	56 Illustrations, color; 36 Illustrations, black and white; XI, 573 p. 92 illus., 56 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	DNA Extraction • electron microscopy • metabolomics analysis • Model organisms • planarian ecology • pluripotent adult stem cells • RNA extraction • single stem cell transplantations
ISBN-10	1-4939-9289-9 / 1493992899
ISBN-13	978-1-4939-9289-8 / 9781493992898
Zustand	Neuware