Fungal Genomics

Methods and Protocols

Ronald P. de Vries, Adrian Tsang, Igor V. Grigoriev (Herausgeber)

Buch | Softcover

291 Seiten

2019 | Softcover reprint of the original 2nd ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-9290-4 (ISBN)

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This volume details protocols covering nearly all aspects of fungal genomics. New and updated chapters guide the reader through experimental genomics, biotechnologies, and the analysis and processing of data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Fungal Genomics : Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Introduction to the Book: Overview of Fungal Genomics.- Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing.- Purification of Fungal High Molecular Weight Genomic DNA from Environmental Samples.- Genome Sequencing.- Methods for Genomic Characterization and Maintenance of Anaerobic Fungi.- Efficient Extraction Method for High Quality Fungal RNA from Complex Lignocellulosic Substrates.- Isolation of high quality RNA for Metatranscriptome Analysis of Lignocellulose Digestion in the Rumen.- Fungal Transcriptomics.- Mass Spectrometrybased Proteomics.- Mass Spectrometry Based Metabolomics.- Genome Editing - CRISPR/cas9.- Evolutionary Adaptation to Generate Mutants.- Genome Assembly.- Fungal Epigenomics: Detection and Analysis.- Fungal Genome Annotation.- Manual Gene Curation and Functional Annotation.- Evaluating Programs for Predicting Genes and Transcripts with RNA-Seq Support in Fungal Genomes.- Genomic Sequence Variation Analysis by Resequencing.- ChIP-seqAnalysis in Neurospora crass.- Fungal Phylogenomics.- Phylogenetic Analysis of Protein Family.- Parasexual Crossings for Bulk Segregant Analysis in Aspergillus Niger To Facilitate Mutant Identification Via Whole Genome Sequencing.

Erscheinungsdatum	22.07.2019
Reihe/Serie	Methods in Molecular Biology ; 1775
Zusatzinfo	48 Illustrations, color; 9 Illustrations, black and white; XI, 291 p. 57 illus., 48 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Mykologie
Schlagworte	ChIP-chip • ChIP-seq analysis • DNA preparation • Genome sequencing • Mass Spectrometry
ISBN-10	1-4939-9290-2 / 1493992902
ISBN-13	978-1-4939-9290-4 / 9781493992904
Zustand	Neuware