Chlamydia trachomatis
Humana Press Inc. (Verlag)
978-1-4939-9693-3 (ISBN)
This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.
Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA).- Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue.- Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae.- Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay.- Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth.- Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples.- Multi Locus Sequence Typing (MLST) of Chlamydiales.- Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method.- Dual RNA-Seq of Chlamydia and Host Cells.- Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection.- Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions.- Mutagenesis of Chlamydia trachomatis Using TargeTron.- Forward and Reverse Genetic Analysis of Chlamydia.- A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions.- Rodent Infections for Chlamydia spp..- Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy.- Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane.- High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis.
| Erscheinungsdatum | 23.08.2019 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 2042 |
| Zusatzinfo | 26 Illustrations, color; 7 Illustrations, black and white; XI, 289 p. 33 illus., 26 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
| Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Technik | |
| Schlagworte | Bacterial pathogens • Bacterium biology • C. trachomatis • drug discovery • drug resistance • treatment regimens |
| ISBN-10 | 1-4939-9693-2 / 1493996932 |
| ISBN-13 | 978-1-4939-9693-3 / 9781493996933 |
| Zustand | Neuware |
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