Immunophenotyping

Methods and Protocols

Buch | Hardcover

356 Seiten

2019 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9649-0 (ISBN)

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This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolvingfield.

High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical Guidebook.- Immunophenotyping by Mass Cytometry.- Fluorescent Cell Barcoding for Immunophenotyping.- Immunophenotyping using Dried and Lyophilized Reagents.- Guidelines for Gating Flow Cytometry Data for Immunological Assays.- Strategies and Techniques for NK Cell Phenotyping.- Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose Tissue.- Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells.- Immunophenotyping of Human Regulatory T Cells.- Immunophenotyping of Human Innate Lymphoid Cells.- Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis.- Immunophenotyping of Circulating Endothelial Cell and Endothelial Microparticles.- Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells.- Quality Control of Immunophenotyping.- Immunophenotyping of Acute Myeloid Leukemia.- Immunophenotyping of Acute Lymphoblastic Leukemia.- Clinical Flow Cytometry Testing in Chronic Lymphocytic Leukemia.- Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH).

Erscheinungsdatum	04.10.2019
Reihe/Serie	Methods in Molecular Biology ; 2032
Zusatzinfo	79 Illustrations, color; 15 Illustrations, black and white; X, 356 p. 94 illus., 79 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	B lymphocytes • fluorescence-based cytometers • Human Stem Cells • intracellular cytokines • Leukemias • lymphoid cells • lyophilized reagents • Mass cytometry • T regulatory cells
ISBN-10	1-4939-9649-5 / 1493996495
ISBN-13	978-1-4939-9649-0 / 9781493996490
Zustand	Neuware