Genotoxicity Assessment

Methods and Protocols

Alok Dhawan, Mahima Bajpayee (Herausgeber)

Buch | Hardcover

378 Seiten

2019 | 2nd ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9645-2 (ISBN)

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This current edition explores new tests for genotoxicity testing, along with other well-known techniques. This will further help in our understanding of the genotoxic effects of chemicals. The book has different sections dealing with various assays for gene mutation, chromosomal abnormalities, primary DNA damage, etc. It also delves into plant models, animals and their alternates, as well as in silico approaches for genetic toxicology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Genotoxicity Assessment: Methods and Protocols, Second Edition serves as a highly useful and ready resource for research students and scientists working in regulatory toxicology as well as biomedical, biochemical, and pharmaceutical sciences.

In Vitro Mouse Lymphoma Cell (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay.- Erythrocyte-Based Pig-a Gene Mutation Assay.- Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes.- In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests.- Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biological Dosimetry.- Chromosomal Aberration Test in Human Lymphocytes.- In Vivo Micronucleus Assay in Mouse Bone Marrow.- Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cells.- Micronucleus Analysis by Flow Cytometry.- Hen’s Egg Test for Micronucleus Induction (HET-MN).- Comparative Genomic Hybridization (CGH) in Genotoxicology.- The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage.- The Comet Assay in Human Biomonitoring.- The Comet Assay in Marine Animals.- Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers.- Detection of DNA Double Strand Breaks Using Pulse Field Gel Electrophoresis.- Detection of UV-Induced Thymine Dimers.- Micronucleus Assay with Tetrad Cells of Tradescantia.- The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila.- In Silico Approaches in Predictive Genetic Toxicology.

Erscheinungsdatum	20.09.2019
Reihe/Serie	Methods in Molecular Biology ; 2031
Zusatzinfo	57 Illustrations, color; 37 Illustrations, black and white; XI, 378 p. 94 illus., 57 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie
	Medizin / Pharmazie ► Pharmazie
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Studium ► 2. Studienabschnitt (Klinik) ► Pharmakologie / Toxikologie
	Naturwissenschaften ► Chemie ► Technische Chemie
	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	chromosomal abnormalities • Computational predictive models • DNA Damage • Genetic toxicology • Mutation assays • toxicogenomics
ISBN-10	1-4939-9645-2 / 1493996452
ISBN-13	978-1-4939-9645-2 / 9781493996452
Zustand	Neuware