Plant Chromatin Dynamics
Humana Press Inc. (Verlag)
978-1-4939-8451-0 (ISBN)
Authoritative and cutting-edge, Plant Chromatin Dynamics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Section Overview: Profiling Developmentally and Environmentally Controlled Chromatin Reprogramming.- Profiling DNA Methylation using Bisulfite Sequencing (BS-Seq).- Bisulfite Sequencing using Small DNA Amounts.- Identification of Differentially Methylated Regions in the Genome of Arabidopsis thaliana.- A Rapid and Efficient ChIP Protocol to Profile Chromatin Binding Proteins and Epigenetic Modifications in Arabidopsis.- Sequential ChIP Protocol for Profiling Bivalent Epigenetic Modifications (ReChIP).- A Method to Identify Nucleolus-Associated Chromatin Domains (NADs).- Cell Type-Specific Profiling of Chromatin Modifications and Associated Proteins.- Mapping of Histone Modifications in Plants by Tandem Mass Spectrometry.- Histone H1 Purification and Post-Translational Modification Profiling by High–Resolution Mass Spectrometry.- Profiling Nucleosome Occupancy by MNase-seq: Experimental Protocol and Computational Analysis.- Identification of Open Chromatin Regions in Plant Genomes usingATAC-Seq.- Section Overview: Unravelling the Complex Epigenetic Mechanisms that Regulate Gene Activity.- Technical Review: A Hitchhiker’s Guide to Chromosome Conformation Capture.- 3C in Maize and Arabidopsis.- Profiling Histone Modifications in Synchronised Floral Tissues for Quantitative Resolution of Chromatin and Transcriptome Dynamics.- De Novo Identification of sRNA Loci and Non-Coding RNAs by High-Throughput Sequencing.- Identification of in planta Protein-Protein Interactions using IP-MS.- RNA Immunoprecipitation Protocol to Identify Protein-RNA Interactions in Arabidopsis thaliana.- In vitro Assays to Measure Histone Methyltransferase Activity using Different Chromatin Substrates.- Identification of Parent-of-Origin-Dependent QTLs using Bulk-Segregant Sequencing (Bulk-Seq).- QTLepi mapping in Arabidopsis thaliana.- Section Overview: a Compendium of Methods to Analyze the Spatial Organization of Plant Chromatin.- Localisation of Chromatin Marks in Arabidopsis EarlyEmbryos.- Cell-type Specific Chromatin Analysis in Whole-mount Plant Tissues by Immunostaining.- Measuring Dynamics of Histone Proteins by Photobleaching in Arabidopsis Roots.- Fluorescence In Situ Hybridization (FISH) and Immunolabeling on 3D Preserved Nuclei.- High-Affinity LNA/DNA Mixmer Probes for Detection of Chromosome-Specific Polymorphisms of 5S rDNA Repeats in Arabidopsis thaliana.- A Method for Testing Random Spatial Model on Nuclear Object Distributions.- Technical Review: Cytogenetic Tools for Studying Mitotic Chromosomes.- Technical Review: Microscopy and Image Processing Tools to Analyse Plant Chromatin – practical Considerations.- Automated 3D Gene Position Analysis using a Customized Imaris Plugin: XTFISHInsideNucleus.- Quantitative 3D Analysis of Nuclear Morphology and Heterochromatin Organization from Whole Mount Plant Tissue using NucleusJ.- Transmission Electron Microscopy Imaging to Analyse Chromatin Density Distribution at the Nanoscale Level.
| Erscheint lt. Verlag | 23.8.2018 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1675 |
| Zusatzinfo | 66 Illustrations, color; 24 Illustrations, black and white; XIV, 659 p. 90 illus., 66 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
| ISBN-10 | 1-4939-8451-9 / 1493984519 |
| ISBN-13 | 978-1-4939-8451-0 / 9781493984510 |
| Zustand | Neuware |
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