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Investigation of Nanoscopic Dynamics and Potentials by Interferometric Scattering Microscopy - Jaime Ortega Arroyo

Investigation of Nanoscopic Dynamics and Potentials by Interferometric Scattering Microscopy

Buch | Softcover
XXXVI, 142 Seiten
2019 | 1. Softcover reprint of the original 1st ed. 2018
Springer International Publishing (Verlag)
978-3-030-08368-7 (ISBN)
CHF 164,75 inkl. MwSt
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This thesis offers a unique guide to the development and application of ultrasensitive optical microscopy based on light scattering. Divided into eight chapters, it covers an impressive range of scientific fields, from basic optical physics to molecular biology and synthetic organic chemistry. Especially the detailed information provided on how to design, build and implement an interferometric scattering microscope, as well as the descriptions of all instrumentation, hardware interfacing and image processing necessary to achieve the highest levels of performance, will be of interest to researchers now entering the field.

Jaime Ortega-Arroyo is currently a postdoctoral researcher in Romain Quidant's plasmon nano-optics group at the ICFO (Institute of Photonic Sciences) in Barcelona, Spain. His current research focuses on combining ultrasensitive light microscopy with plasmon-assisted optofluidics. He received his BSc in Chemistry and Mathematics from the University of British Columbia, Vancouver, Canada in 2010, and his PhD in Physical and Theoretical Chemistry from the University of Oxford, UK in 2015, under the guidance of Phillip Kukura.

Introduction.- Non-fluorescent single-molecule approaches to optical microscopy.- Experimental Methods.- Anomalous diffusion due to interleaflet coupling and molecular pinning.- Structural dynamics of myiosin 5a.- All optical label-free detection, imaging and tracking of single proteins.- Single-molecule chemical dynamics: direct observation of physical autocatalysis.- Outlook.

Erscheinungsdatum
Reihe/Serie Springer Theses
Zusatzinfo XXXVI, 142 p. 53 illus., 25 illus. in color.
Verlagsort Cham
Sprache englisch
Maße 155 x 235 mm
Gewicht 284 g
Themenwelt Naturwissenschaften Chemie Physikalische Chemie
Schlagworte High Speed Single-particle Tracking • Interferometric Scattering Microscopy (iSCAT) • Label-free Imaging • Label-free Single-protein Detection • Membrane biophysics • Myosin 5a Dynamics • Physical Autocatalysis • Single-molecule Approaches to Optical Microscopy • Ultrasensitive Optical Microscopy
ISBN-10 3-030-08368-3 / 3030083683
ISBN-13 978-3-030-08368-7 / 9783030083687
Zustand Neuware
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