Sertoli Cells

Methods and Protocols

Marco G. Alves, Pedro F. Oliveira (Herausgeber)

Buch | Softcover

297 Seiten

2019 | Softcover reprint of the original 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-8543-2 (ISBN)

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This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture. Prepared for non-specialists as well as experienced researchers that may be interested in a multidisciplinary approach to study these cells, the book examines these remarkable cells that present some unique features that go far beyond the physical and nutritional support of spermatogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Sertoli Cells: Methods and Protocols serves as a valuable resource for experienced researchers in reproductive biology, particularly andrologists, but also for future investigators and young students starting to study these extraordinary cells.

Establishment of Primary Culture of Sertoli Cells.- Evaluation of the Purity of Sertoli Cell Primary Cultures.- Preparation of Testicular Samples for Histology and Immunohistochemistry.- Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age.- Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy.- Sertoli Cells Preparation for Co-Immunoprecipitation.- Profiling Signaling Proteins in Sertoli Cells by Co-Immunoprecipitation.- Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy.- A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells.- Assessing Autophagy in Sertoli Cells.- Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells.- Assessing Sertoli Cells Metabolic Activity.- Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy.- Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs.- Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice.- Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of the Actin, and Microtubule-Based Cytoskeletons.- Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay.- Computational Methods Involved in Evaluating the Toxicity of the Reproductive Roxicants in Sertoli Cell.- A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells.- Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing.

“The pictures illustrating the book are all of high quality and the readers will be satisfied and certainly not disappointed while running through the chapters: exactly in the same manner you cannot stop of eating chapters or cherries, one is calling the other!” (CarloAlberto Redi, European Journal of Histochemistry, Vol. 63 (01), 2019)

Erscheinungsdatum	01.04.2019
Reihe/Serie	Methods in Molecular Biology ; 1748
Zusatzinfo	37 Illustrations, color; 12 Illustrations, black and white; XIII, 297 p. 49 illus., 37 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Andrology • Cell Culture • Reproductive Biology • Somatic testicular cells • Spermatogenesis
ISBN-10	1-4939-8543-4 / 1493985434
ISBN-13	978-1-4939-8543-2 / 9781493985432
Zustand	Neuware