Single Cell Methods
Humana Press Inc. (Verlag)
978-1-4939-9239-3 (ISBN)
Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.
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Setting Up a Single-Cell Genomic Laboratory.- Tissue Handling and Dissociation for Single-Cell RNA-seq.- Full-Length Single-Cell RNA-Sequencing with Smart-seq2.- CEL-Seq2 – Single Cell RNA Sequencing by Multiplexed Linear Amplification.- Single-Cell RNA-seq by Multiple Annealing and Tailing Based Quantitative Single-Cell RNA-seq (MATQ-seq).- Single-Cell RNA Sequencing with Drop-Seq.- Chromium 10X Single Cell 3’ mRNA Sequencing of Tumor Infiltrating Lymphocytes.- Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA-Sequencing.- Single-Cell Tagged Reverse Transcription (STRT-seq).- Single-Cell RNA-Sequencing of Peripheral Blood Mononuclear Cells with ddSEQ.- High Throughput Single Cell Real-Time Quantitative PCR Analysis.- Single-Cell Dosing and mRNA Sequencing of Suspension and Adherent Cells Using the Polaris™ System.- Targeted TCR Amplification from Single-Cell cDNA Libraries.- Sequencing the Genomes of Single Cells.- Studying DNA Methylation in Single Cell Format with scBS-seq.- Single Cell 5fC Sequencing.- ChIPmentation for Low-Input Profiling of In Vivo Protein-DNA Interactions.- Immuno-Phenotyping of Human Peripheral Blood Mononuclear Cells by Mass Cytometry.- Classification of the Immune Composition in the Tumor Infiltrate.- Combined Genome and Transcriptome (G&T) Sequencing of Single Cells.- Simultaneous Pofiling of mRNA Transcriptome and DNA Methylome from a Single Cell.- Simultaneous Targeted Detection of Proteins and RNAs in Single Cells.- CRISPR-Screening in Single Cells.- Single Cell Live Imaging.- Differential Expression Analysis in Single Cell Transcriptomics.- A Bioinformatic Toolkit for Single-Cell mRNA Analysis.
Erscheinungsdatum | 17.05.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 1979 |
Zusatzinfo | 78 Illustrations, color; 9 Illustrations, black and white; XVI, 459 p. 87 illus., 78 illus. in color. With online files/update. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | Crispr-Screening • Drop-seq • FISSEQ • PCR • sc-ChIP-seq |
ISBN-10 | 1-4939-9239-2 / 1493992392 |
ISBN-13 | 978-1-4939-9239-3 / 9781493992393 |
Zustand | Neuware |
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