Candida Species

Methods and Protocols

Richard Calderone, Ronald Cihlar (Herausgeber)

Buch | Softcover

240 Seiten

2019 | Softcover reprint of the original 1st ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-7980-6 (ISBN)

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This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Candida Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.

How to use the Candida Genome Database.- Transcriptional Profiling of Candida albicans in the Host.- SRYTH: A New Yeast Two-Hybrid Method.- Chromatin immunoprecipitation (ChIP) assay in Candida albicans .- Assessing mitochondrial functions in Candida albicans.- Imaging Candida infections in the host.- Identification of Secreted Candida Proteins using Mass Spectrometry.- In vitro models for Candida Biofilm Development.- The Fungal Biome of the Oral Cavity.- Experimental models of C. albicans-streptococcal Co-infection.- Staphylococcus-Candida interaction models: Antibiotic Resistance Testing and Host Interaction.- Genetic Screens for Determination of Mechanism of Action.- Microbroth Dilution Susceptibility Testing of Candida Species.- Biofilms and Antifungal Susceptibility Testing.- Typing Candida Species using Microsatellite Length Polymorphism and Multilocus Sequence Typing.- Diagnostic Methods for Detection of Blood-borne Candidiasis.

Erscheinungsdatum	20.12.2018
Reihe/Serie	Methods in Molecular Biology ; 1356
Zusatzinfo	49 Illustrations, color; 6 Illustrations, black and white; XI, 240 p. 55 illus., 49 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	antifungal drug discovery • C. albicans • fungal molecular biology • methodological advances • pathogenesis
ISBN-10	1-4939-7980-9 / 1493979809
ISBN-13	978-1-4939-7980-6 / 9781493979806
Zustand	Neuware