Viral Vectors for Gene Therapy
Humana Press Inc. (Verlag)
978-1-4939-9064-1 (ISBN)
Comprehensive and authoritative, Viral Vectors for Gene Therapy: Methods and Protocols is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments.
Basic Concepts in Viral Vector-Mediated Gene Therapy.- Design and Assembly of CRISPR/Cas9 Lentiviral and rAAV Vectors for Targeted Genome Editing.- Design, Construction, and Application of Transcription Activation-Like Effectors.- Practical Considerations for the use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain.- AAV Production using Baculovirus Expression Vector System.- Multimodal Production of Adeno-Associated Virus.- Generation of High Titer Pseudotyped Lentiviral Particles.- A Scalable Lentiviral Vector Production and Purification Method using Mustang Q Chromatography and Tangential Flow Filtration.- Current Use of Adenovirus Vectors and their Purification Methods.- Construction of Oncolytic Herpes Simplex Virus with Therapeutic Genes of Interest.- Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes.- AAV Mediated Gene Delivery to the Mouse Liver.- Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse.- Gene Transfer to Mouse Kidney In Vivo.- Co-Delivery of a Short-Hairpin RNA and a shRNA-Resistant Replacement Gene with Adeno-Associated Virus: An Allele-Independent Strategy for Autosomal Dominant Retinal Disorders.- Localized Intra-Arterial Gene Delivery using AAV.- Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors.- Systemic Delivery of Adeno-Associated Viral Vectors in Mice and Dogs.- Intrathecal Delivery of AAV Vectors in Cynomolgus Macaques for CNS Gene Therapy and Gene Expression Analysis in Microdissected Motor Neurons.- Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice.- Cerebromedullary Cistern Injection of Viral Vectors in Non-Human Primates.
Erscheinungsdatum | 07.03.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 1937 |
Zusatzinfo | 33 Illustrations, color; 13 Illustrations, black and white; XIV, 328 p. 46 illus., 33 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Biomedizin |
Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin | |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-4939-9064-0 / 1493990640 |
ISBN-13 | 978-1-4939-9064-1 / 9781493990641 |
Zustand | Neuware |
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