Oligodendrocytes

Methods and Protocols

David A. Lyons, Linde Kegel (Herausgeber)

Buch | Hardcover

411 Seiten

2019 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9070-2 (ISBN)

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This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.

Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System.- Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing.- Myelin: Methods for Purification and Proteome Analysis.- In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells.- Isolation and Culture of Oligodendrocytes.- A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation.- Oligodendrocyte-Neuron Myelinating Co-Culture.- Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier.- Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices.- Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord.- Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination.- Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination.- A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation.- Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis.- Conditional Mutagenesis in Oligodendrocyte Lineage Cells.- Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage.- The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination.- Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease.- A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes.- Transmission Electron Microscopy of Oligodendrocytes and Myelin.- Toxin-Based Models to Investigate Demyelination and Remyelination.- Magnetic Resonance techniques for Imaging White Matter.

Erscheinungsdatum	15.03.2019
Reihe/Serie	Methods in Molecular Biology ; 1936
Zusatzinfo	55 Illustrations, color; 10 Illustrations, black and white; XII, 411 p. 65 illus., 55 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Naturwissenschaften ► Biologie ► Zoologie
ISBN-10	1-4939-9070-5 / 1493990705
ISBN-13	978-1-4939-9070-2 / 9781493990702
Zustand	Neuware