Phototropism

Methods and Protocols

Buch | Hardcover

240 Seiten

2019 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9014-6 (ISBN)

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This book aims to promote studies on the entire spectrum of phototropic phenomena in higher and lower plants and fungi. Chapters detail phototropism in many plant species induced by far-red, red, blue and UV lights. They also include methods for auxin biology and analysis of cytoskeleton and phototropin. In addition, the use of grafting, spaceflight experiment and image analysis in tropism study is provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Phototropism: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Physiological Characterization of Phototropism in Arabidopsis Seedlings.- Assessing Negative and Positive Phototropism in Lianas.- Determination of Phototropism and Polarotropism in Fern Protonemal Cells.- Assays of Protonemal Growth Responses in Physcomitrella patens under Blue- and Red-light Stimuli.- An Experimental System for Examining Phototropic Response of Gametophytic Shoots in the Moss Physcomitrella patens.- Observation of Phototropic Responses in the Liverwort Marchantia polymorpha.- Measurement of Phototropism of the Sporangiophore of Phycomyces blakesleeanus.- Quantitative Analysis of Tip Growth, Phototropic Responses and Other Blue Light-Dependent Photoresponses of Vaucheria.- A Simple Procedure to Observe Phototropic Responses in the Red Seaweed Pyropia yezonensis.- Determination of Phototropism by UV-B Radiation.- New-Generation Chemical Tools for the Manipulation of Auxin Biosynthesis, Action, and Transport.-Determination of Auxin Flow during Phototropic Responses Using Fluorescent Auxin Analogs.- Immunolocalization of IAA Using an Anti-IAA-C Antibody Raised against Carboxyl-Linked IAA.- Measurements of Photoreaction and Kinase Activity of Phototropin, a Photoreceptor Protein for Tropic Response in Plants; Involvement of Kinase Activity in the Photosensitivity of Tropic Response.- Cytoskeleton Observation in Phototropism and Polarotropism of Adiantum Protonema.- Grafting as a Tool for Study of Phototropism in Phycomyces.- Preparation of a Spaceflight Experiment to Study Tropisms in Arabidopsis Seedlings on the International Space Station.- How to Investigate the Role of the Actin-Myosin Cytoskeleton in Organ Straightening.- Quantitative Measurements of Curvature along the Growth Axis in Tropic Responses Using Free Software Environments.

Erscheinungsdatum	19.02.2019
Reihe/Serie	Methods in Molecular Biology ; 1924
Zusatzinfo	47 Illustrations, color; 42 Illustrations, black and white; X, 240 p. 89 illus., 47 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Schlagworte	auxin • Curvature • Cytoskeleton • immunohistochemistry • microelectrode
ISBN-10	1-4939-9014-4 / 1493990144
ISBN-13	978-1-4939-9014-6 / 9781493990146
Zustand	Neuware