Bone Research Protocols

Aymen I. Idris (Herausgeber)

Buch | Hardcover

670 Seiten

2019 | 3rd ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-8996-6 (ISBN)

Infos

The third edition of this book provides a large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research.

Inhalt

Isolation and Generation of Osteoclasts.- Isolation and Generation of Osteoblasts.- Isolation, Purification, Generation, and Culture of Osteocytes.- Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo.- 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies.- Using Cell and Organ Culture Models to Analyse Responses of Bone Cells To Mechanical Stimulation.- The ex vivo Organ Culture of Bone.-  Ex-Vivo Model Systems of Cancer-bone Cell Interactions.- Mechanical Properties of Bone ex vivo.- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation.- Analysis of Transcriptional Regulation in Bone Cells.- Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization.- Ovariectomy/Orchiectomy in Rodents.- In Vivo Models of Rheumatoid Arthritis.- Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus.- Models of Prostate Cancer Bone Metastasis.-Animal Models of Breast Cancer Bone Metastasis.- Murine Models of Bone Sarcomas.- Mouse Models of Melanoma Bone Metastasis.- Animal Models of Multiple Myeloma.- The Calvarial Injection Assay.- In Vivo Models of Mechanical Loading.- Animal Models for the Study of Bone Derived Pain.- Histomorphometry in Rodents.- Immunostaining of Skeletal Tissues.- Techniques for the Study of Apoptosis in Bone.- Live Cell Imaging of Bone Cell and Organ Cultures.- Analysis of Bone Architecture in Rodents using micro Computed Tomography.- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents.- Quantitative X-ray Imaging of Mouse Bone by Faxitron.- Scanning Electron Microscopy of Bone.- Transmission Electron Microscopy of Bone.- Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy.- Fourier Transform Infrared Imaging of bone.- Raman Microscopy and Bone.

Produkt-Details

• Erscheinungsdatum: 05.02.2019
• Reihe/Serie: Methods in Molecular Biology ; 1914
• Zusatzinfo: 121 Illustrations, color; 51 Illustrations, black and white; XV, 670 p. 172 illus., 121 illus. in color.
• Verlagsort: Totowa, NJ
• Sprache: englisch
• Maße: 178 x 254 mm
• Themenwelt: Medizinische Fachgebiete ► Chirurgie ► Unfallchirurgie / Orthopädie
• Themenwelt: Medizin / Pharmazie ► Studium
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Schlagworte: Oestrogen deficiency • Osteoclasts • osteocytes • Rheumatoid Arthritis • Stem Cells
• ISBN-10: 1-4939-8996-0 / 1493989960
• ISBN-13: 978-1-4939-8996-6 / 9781493989966
• Zustand: Neuware