Hepatitis C Virus Protocols
Humana Press Inc. (Verlag)
978-1-4939-8975-1 (ISBN)
Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field.
Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus.- Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV.- Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR).- Establishment of Replication-competent HCV Strain with Minimum Modifications.- Full-length Open Reading Frame Amplification of Hepatitis C Virus.- InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones.- Production and Purification of Cell Culture Hepatitis C Virus.- Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture.- Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing.- A Cell Culture Model for Persistent HCV Infection.- Expression Cloning of Host Factors Required for the HCV Replication Cycle.- Application of Deep Mutational Scanning in Hepatitis C Virus.- Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals.- Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay.- Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells.- Functional Characterization of Apolipoproteins in the HCV Life Cycle.- Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes.- Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins.- Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis.- Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells.- Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact.- Complement Regulation and Immune Evasion by Hepatitis C Virus.- Direct ex vivo Functional Analysis of HCV-specific T Cells.- Simultaneous Analysis of HCV-specific CD4+ and CD8+ T Cells by Multi-color Flow Cytometry.- Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology. -Isolation of HCV Neutralizing Antibodies by Yeast Display.- Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA.- In vitro Neutralization Assay using Cultured Hepatitis C Virus.- Standardized Method for the Study of Antibody Neutralization of HCV pseudoparticles (HCVpp).- Detection of Antigen-specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice.- Mice with Chimeric Human Livers and Their Applications.- Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies.
| Erscheinungsdatum | 18.01.2019 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1911 |
| Zusatzinfo | 47 Illustrations, color; 28 Illustrations, black and white; XIX, 508 p. 75 illus., 47 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizinische Fachgebiete ► Innere Medizin ► Hepatologie |
| Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin | |
| Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | Flaviviridae • Hepacivirus • pegiviruses • pseudotype HCV • Virology |
| ISBN-10 | 1-4939-8975-8 / 1493989758 |
| ISBN-13 | 978-1-4939-8975-1 / 9781493989751 |
| Zustand | Neuware |
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