Super-Resolution Microscopy

Methods and Protocols

Buch | Softcover

262 Seiten

2018 | Softcover reprint of the original 1st ed. 2017
Humana Press Inc. (Verlag)
978-1-4939-8435-0 (ISBN)

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This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.

Super-Resolution Microscopy Techniques and their Potential for Applications in Radiation Biophysics.- Managing the Introduction of Super-Resolution Microscopy into a Core Facility.- Live-Cell STED Imaging with the HyPer2 Biosensor.- Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope.- Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo.- STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells.- Four Channel Super-Resolution Imaging by 3-D Structured Illumination.- Correlative SIM-STORM Microscopy.- Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples.- Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles.- Two-Color Single-Molecule Tracking in LiveCells.- Fully Automated Targeted Confocal and Single Molecule Localization Microscopy.- Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy.- Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells.- Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and their use in Cellular Imaging Experiments.- Measuring Nanometer Distances between Fluorescent Labels Step-by-Step.- Correlative Single Molecule Localization Microscopy and Confocal Microscopy.- Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells.- In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT.- Super-Resolution High Content Screening and Analysis.

“The aim is to provide a current overview of the advanced technologies and methods in super‐resolution microscopy. The book is written for novices andexperienced investigators. It will be of much help for graduate students and postdoctoral students interested in super-resolution microscopy. The author is a credible authority in the field of super-resolution microscopy. … This book covers advanced, cutting-edge techniques related to super-resolution microscopy. The intended audience will find it of immense help.” (Omer Iqbal, Doody's Book Reviews, February, 2018)

Erscheinungsdatum	27.08.2018
Reihe/Serie	Methods in Molecular Biology ; 1663
Zusatzinfo	37 Illustrations, color; 11 Illustrations, black and white; XI, 262 p. 48 illus., 37 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Gesundheitsfachberufe ► MTA - Radiologie
	Medizinische Fachgebiete ► Radiologie / Bildgebende Verfahren ► Radiologie
	Medizin / Pharmazie ► Studium ► 1. Studienabschnitt (Vorklinik)
	Naturwissenschaften ► Biologie ► Biochemie
ISBN-10	1-4939-8435-7 / 1493984357
ISBN-13	978-1-4939-8435-0 / 9781493984350
Zustand	Neuware