Antimicrobial Peptides
Humana Press Inc. (Verlag)
978-1-4939-8287-5 (ISBN)
Authoritative and cutting-edge, Antimicrobial Peptides: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Antimicrobial Peptides: An Introduction.- Tools for designing amphipathic helical antimicrobial peptides.- Chemical Synthesis of Antimicrobial Peptides.- Microwave-Assisted Synthesis of Antimicrobial Peptides.- High Performance Liquid Chromatography and Mass Spectrometry-Based Design of Protolytically Stable Antimicrobial Peptides.- Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR.- Mass spectrometry approaches for determining the structure of antimicrobial peptides.- Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptides-Lipid Bilayers Interactions.- Calorimetry methods to study membrane interactions and perturbations induced by antimicrobial host defense peptides.- Fluorescence and absorbance spectroscopy methods to study membrane perturbations by antimicrobial host defense peptides.- Applying Fluorescence Correlation Spectroscopy to InvestigatePeptide-Induced Membrane Disruption.- Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action.- Using Confocal Microscopy and Computational Modeling to Investigate the Cell Penetrating Properties of Antimicrobial Peptides.- Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA.- Protocols for studying the interaction of MSI-78 with the Membranes of Whole Gram-positive and Gram-negative Bacteria by NMR.- Preparation of membrane models of Gram-negative bacteria and their interaction with Antimicrobial Peptides studied by CD and NMR.- Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells using Circular Dichroism.- Studying the Mechanism of Membrane Permeabilization induced by Antimicrobial Peptides using Patch-clamp Techniques.- Assays for Identifying Inducers of the Antimicrobial Peptide LL-37.- Methods for Elucidating the Mechanism of Action of Proline-rich and other Non-lytic Antimicrobial Peptides.- The Interaction of Antimicrobial Peptides with the Membrane and Intracellular Targets of Staphylococcus Aureus Investigated by ATP-leakage, DNA-binding Analysis and the Expression of a LexA Controlled Gene, recA.- Methods for Investigating Biofilm Inhibition and Degradation by Antimicrobial Peptides.- Protocols for Studying Inhibition and Eradication of Bacterial Biofilms by Antimicrobial Peptides.- Protocols for Studying Antimicrobial Peptides (AMPs) as Anti-cancer Agents.- Using Oral and Colon Cancer Cells for studying the Anti-cancer properties of Antimicrobial peptides.- Using Disease-Associated Enzymes to Activate Antimicrobial Peptide Prodrugs.- Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization.- Protocols for Screening Antimicrobial Peptides That Influence Virulence Gene Expression in Staphylococcus aureus.- Methods for in vitro Analysis of Antimicrobial Activity and Toxicity of Anti-keratitis Peptides: Bacterial Viability in tears, MTT and TNF-a release assays.- Methods for In Vivo/ex-vivo Analysis of Antimicrobial Peptides in Bacterial Keratitis: siRNA Knockdown, Colony Counts, Myeloperoxidase, Immunostaining, and RT-PCR Assays.- Haemolytic Activity of Antimicrobial Peptides.
Erscheinungsdatum | 18.08.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1548 |
Zusatzinfo | 49 Illustrations, color; 38 Illustrations, black and white; XIV, 439 p. 87 illus., 49 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Schlagworte | Antimicrobial peptides • biological activities of AMPs • fluorescence spectroscopy methods • interaction of AMPs with bacteria • interaction of AMPs with model systems • patch-clamp techniques • synthesis |
ISBN-10 | 1-4939-8287-7 / 1493982877 |
ISBN-13 | 978-1-4939-8287-5 / 9781493982875 |
Zustand | Neuware |
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