Plant Cytogenetics
Humana Press Inc. (Verlag)
978-1-4939-8097-0 (ISBN)
This volume covers a
range of methods used in plant cytogenetics, beginning with basic analysis of
chromosomes and visualizing gene locations, to manipulating and dissecting
chromosomes, and then focusing on less understood features of chromosomes such
as recombination initiation sites and epigenomic marks. The methods described
in Plant Cytogenetics: Methods and Protocols build on each other and
provide, those new to the field, with a comprehensive platform to support their
research endeavours, while also introducing advanced techniques to experienced
researchers. Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting
edge and thorough, Plant Cytogenetics: Methods and Protocols, is a
valuable resource for anyone who is interested in the diverse and wonderfully
complex field of cytogenetics.
Shahryar Kianian Research Leader USDA-ARS Cereal Disease Laboratory University of Minnesota St. Paul, MN 55108 Email: Shahryar.Kianian@ars.usda.gov Penny M Avoles Kianian Alderman Hall, St. Paul, MN 55108 Email:P.Kianian@gmail.com
C-Banding of Plant Chromosomes.- Chromosome Painting by GISH and Multi-Color
FISH.- Fluorescent In Situ Hybridization on Extended Chromatin Fibers for
High-Resolution Analysis of Plant Chromosomes.- Tyramide Signal Amplification: Fluorescence
In Situ Hybridization for Identifying
Homologous Chromosomes.- Localization
of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH.- Immunolabeling
and In Situ Labelling of Isolated
Plant Interphase Nuclei.- Manipulation
of Homologous Chromosome Recombination in Wheat.- Dissecting
Plant Chromosomes by the Use of Ionizing Radiation.- Optical
Nano-Mapping and Analysis of Plant Genomes.- Flow
Sorting Plant Chromosomes.- Construction
of BAC Libraries from Flow-Sorted Chromosomes.- The
Chromosome Microdissection and Microcloning Technique.- Immunolocalization
on Whole Anther Chromosome Spreads for Male Meiosis.- Mapping
Recombination Initiation Sites Using Chromatin Immunoprecipitation.- Chromatin
Immunoprecipitation to Study Plant Epigenome.
| Erscheinungsdatum | 25.08.2018 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1429 |
| Zusatzinfo | 32 Illustrations, color; 8 Illustrations, black and white; XII, 198 p. 40 illus., 32 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
| Schlagworte | chromosome behavior • cytogenetic • dissecting chromosomes • epigenomic marks • manipulation of chromosomes • recombination initiation sites • visualizing gene locations • whole genome analysis |
| ISBN-10 | 1-4939-8097-1 / 1493980971 |
| ISBN-13 | 978-1-4939-8097-0 / 9781493980970 |
| Zustand | Neuware |
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