Heterologous Gene Expression in E.coli
Humana Press Inc. (Verlag)
978-1-4939-8328-5 (ISBN)
Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.
Recombinant Protein Expression in E. coli: A Historical Perspective.- N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools.- Harnessing the Profinity eXactTM System for Expression and Purification of Heterologous Proteins in E. coli.- ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences.- Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters.- Optimization of Membrane Protein Production Using Titratable Strains of E. coli.- Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) or pReX and GFP-Fusions.- High Yield of Recombinant Protein in Shaken E. coli Cultures with Enzymatic Glucose Release Medium EnPresso B.- A Generic Protocol for Purifying Disulphide-Bonded Domains and Random Protein Fragments Using FusionProteins with SUMO3 and Cleavage by SenP2 Protease.- A Strategy for Production of Correctly Folded Disulfide-Rich Peptides in the Periplasm of E. coli.- Split GFP Complementation as Reporter of Membrane Protein Expression and Stability in E. coli: A Tool to Engineer Stability in a LAT Transporter.- Acting on Folding Effectors to Improve Recombinant Protein Yields and Functional Quality.- Protein Folding Using a Vortex Fluidic Device.- Removal of Affinity Tags with TEV Protease.- Generation of Recombinant N-Linked Glycoproteins in E. coli.- Production of Protein Kinases in E. coli.- Expression of Prokaryotic Integral Membrane Proteins in E. coli.- Multiprotein Complex Production in E. coli: The SecYEG-SecDFYajC-YidC Holotranslocon.- Membrane Protein Production in E. coli Lysates in Presence of Pre-Assembled Nanodiscs.- Not Limited to E. coli: Versatile Expression Vectors for Mammalian Protein Expression.- A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis.- In Vivo Biotinylation of Antigens in E. coli.- Cold-Shock Expression System in E. coli for Protein NMR Studies.- High-Throughput Production of Proteins in E. coli for Structural Studies.- Mass Spectrometric Analysis of Proteins.- How to Determine Interdependencies of Glucose and Lactose Uptake Rates for Heterologous Protein Production with E. coli.- Interfacing Biocompatible Reactions with Engineered Escherichia coli.
| Erscheinungsdatum | 25.08.2018 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1586 |
| Zusatzinfo | 55 Illustrations, color; 37 Illustrations, black and white; XIV, 429 p. 92 illus., 55 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
| Schlagworte | Escherichia coli • Expression screening • High-throughput cloning • Protein Purification • recombinant proteins • Solubility enhancement • structural biology |
| ISBN-10 | 1-4939-8328-8 / 1493983288 |
| ISBN-13 | 978-1-4939-8328-5 / 9781493983285 |
| Zustand | Neuware |
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