CD95
Humana Press Inc. (Verlag)
978-1-4939-8298-1 (ISBN)
Authoritative and practical, CD95: Methods and Protocols aims to foster original research on CD95/CD95L couple.
Production of the Non-apoptotic Metalloprotease-cleaved CD95L and its Cytotoxic Recombinant Counterpart Designed Ig-CD95L.- CD95 Stimulation with CD95L and DISC Analysis.- Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8.- In vitro Evaluation of the Apoptosis Function in Human Activated T cells.- Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition.- Fluorometric Methods for Detection of Mitochondrial Membrane Depolarization.- Generation and Application of Bioluminescent CD95 Ligand Fusion Proteins.- CD95-mediated Calcium Signaling.- CD95-mediated Proton Regulation.- Study of the CD95-mediated Non-apoptotic Signaling Pathway – PI3K.- Organelle Separation and Cell Signaling.- Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L.- Isolation of Lipid Rafts through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death Receptor Localization in Raft Fractions.- Quantifying CD95/cl-CD95L Implications in CellMechanics And Membrane Tension by Atomic Force Microscopy Based Force Measurements.- Sketching of CD95 Oligomers by In Silico Investigations.- Site-specific Detection of Tyrosine Phosphorylated CD95 Following Protein Separation by Conventional and Phospho-Protein Affinity SDS-PAGE.- Detection of S-acylated CD95 by Acyl-biotin Exchange.- Exploration of Fas S-nitrosylation by the Biotin Switch Assay.- Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L.- Liquid Chromatography-High Resolution Mass Spectrometry Method to Study Sphingolipid Metabolism Changes in Response to CD95L.- CD95 and the MRL-lpr Mouse Model.
Erscheinungsdatum | 16.08.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1557 |
Zusatzinfo | 38 Illustrations, color; 25 Illustrations, black and white; XI, 230 p. 63 illus., 38 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | Apoptosis • Cell Motility • Immunoprecipitation • pathophysiology • TNF Receptor |
ISBN-10 | 1-4939-8298-2 / 1493982982 |
ISBN-13 | 978-1-4939-8298-1 / 9781493982981 |
Zustand | Neuware |
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