Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe
Seiten
1997
|
1997
Springer Berlin (Verlag)
978-3-540-63270-2 (ISBN)
Springer Berlin (Verlag)
978-3-540-63270-2 (ISBN)
Recombinant proteins, produced in cells or microorganisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. However, only recently has the expression system of Schizosaccharomyces pombe as a host become a subject of focus, as it is expected to be profitable for expressing foreign genes. The reason for this is that S. pombe is evolutionarily more advanced than other types of yeast. This book describes the use of S. pombe in basic research and industrial applications.
Characteristics of "host" and "vector". Fission yeast S.pombe: an attractive host for heterologous protein production.- Constitution and development of expression vectors.- Exploiting the thiamine biosynthetic gene nmt1 for regulated expression of heterologous DNAs in fission yeast.- Examples of heterologous protein production in fission yeast.- Overexpression of proteins with seven transmembrane spanning segments.- Targeting and isolationof tagged membrane proteins.- Expression of human cytochrome P450 form in S.pombe.- Human papillomavirus capsid protein-pREP in S.pombe.
| Erscheint lt. Verlag | 17.10.1997 |
|---|---|
| Reihe/Serie | Biotechnology Intelligence Unit |
| Zusatzinfo | XVII, 179 p. 46 illus., 4 illus. in color. |
| Verlagsort | Berlin |
| Sprache | englisch |
| Maße | 210 x 297 mm |
| Gewicht | 450 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | Biotechnology • Cytochrome P450 • Development • Expression • Gene • gene expression • genes • Hefe • Membrane • Membrane Proteins • Microorganism • microorganisms • Protein • proteins • Research • yeast |
| ISBN-10 | 3-540-63270-0 / 3540632700 |
| ISBN-13 | 978-3-540-63270-2 / 9783540632702 |
| Zustand | Neuware |
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