The Bacterial Nucleoid
Humana Press Inc. (Verlag)
978-1-4939-8384-1 (ISBN)
Thorough and cutting-edge, The Bacterial Nucleoid: Methods and Protocols is a valuable resource that provides a wealth of new information about this chromosome.
Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli.- Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes.- Revealing Sister Chromatid Interactions with the loxP/Cre Recombination Assay.- Transposon Insertion Site Sequencing for Synthetic Lethal Screening.- WGADseq: Whole Genome Affinity Determination of Protein-DNA Binding Sites.- High Resolution Chromatin Immunoprecipitation: ChIP-Sequencing.- Generation and Analysis of Chromosomal Contact Maps of Bacteria.- Nucleoid Associated Proteins: Genome Level Occupancy and Expression Analysis.- Isolation and Analysis of RNA Polymerase Supramolecular Complex with Associated Proteins.- A chromosome Co-Entrapment Assay to Study Topological Protein-DNA Interactions.- Tethered Particle Motion Analysis of the DNA Binding Properties of Architectural Proteins.- Biochemical Analysis of Bacterial Condensins.- Exploring Condensins with Magnetic Tweezers.- Applications of Magnetic Tweezers to Studies of NAPs.- A User-Friendly DNA Modeling Software for the Interpretation of Cryo-Electron Microscopy Data.- Multi-Locus Imaging of the E. coli Chromosome by Fluorescent In Situ Hybridization.- Imaging the Cell Cycle of Pathogen E. coli During Growth in Macrophage.- Measuring In Vivo Protein Dynamics throughout the Cell Cycle using Microfluidics.- Imaging of Bacterial Chromosome Organization by 3D Super-Resolution Microscopy.- Sequential Super-Resolution Imaging of Bacterial Regulatory Proteins, the Nucleoid and the Cell Membrane in Single, Fixed E. coli Cells.- Procedures for Model-Guided Data Analysis of Chromosomal Loci Dynamics at Short Time Scales.- Isolation and Characterization of Bacterial Nucleoids in Microuidic Devices.- Modeling Bacterial DNA: Simulation of Self-Avoiding Supercoiled Worm-Like Chains Including Structural Transitions of the Helix.- Molecular Dynamics Simulation of Supercoiled, Knotted and Catenated DNA Molecules, Including Modeling of Action of DNA Gyrase.
Erscheinungsdatum | 09.07.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1624 |
Zusatzinfo | 82 Illustrations, color; 74 Illustrations, black and white; XIV, 374 p. 156 illus., 82 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-4939-8384-9 / 1493983849 |
ISBN-13 | 978-1-4939-8384-1 / 9781493983841 |
Zustand | Neuware |
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