The Golgi Complex
Humana Press Inc. (Verlag)
978-1-4939-8208-0 (ISBN)
Practical and detailed, The Golgi Complex: Methods and Protocols will aid both new and established researchers in the field by providing strong hands-on instructions that can be directly applied to their research programs.
4D Confocal Imaging of Yeast Organelles.- Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons.- Imaging Golgi Outposts in Fixed and Living Neurons.- Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor.- STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells.- Reconstitution of COPI Vesicle and Tubule Formation.- Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules.- Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures.- High Content Analysis of the Golgi Complex by Correlative Screening Microscopy.- Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo.- In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in AdherentMammalian Cell Cultures.- Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function.- Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway.- Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle.- The Role of Lysophospholipid Acyltransferases in the Golgi Complex.- Methods to Purify and Assay Secretory Pathway Kinases.
Erscheinungsdatum | 03.08.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1496 |
Zusatzinfo | 33 Illustrations, color; 11 Illustrations, black and white; XI, 219 p. 44 illus., 33 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | Biochemical reconstitution systems • Cell imaging techniques • Cellular trafficking • Functional organization • Secretory and endocytic pathways |
ISBN-10 | 1-4939-8208-7 / 1493982087 |
ISBN-13 | 978-1-4939-8208-0 / 9781493982080 |
Zustand | Neuware |
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