Bacterial Cell Wall Homeostasis
Humana Press Inc. (Verlag)
978-1-4939-8114-4 (ISBN)
together the most widely used and important protocols currently being employed
in researching and understanding bacterial cell wall homeostasis. Chapters in Bacterial
Cell Wall Homeostasis cover a variety of subjects, such as: modern
microscopy techniques and other biophysical methods used to characterize the
subcellular structure of the bacterial cell wall; high-throughput approaches
that can be used to identify all the genes and proteins that participate in the
correct functioning of an organism’s cell wall; protocols for assaying
individual gene products for specific cell wall functions or identify chemicals
with inhibitory activity against the cell wall; and methods for analyzing the non-protein
components of the cell wall and the increasing use of computational approaches
for predicting and modeling cell wall related functions and processes. Written
in the highly successful Methods in Molecular Biology series format,
chapters include introduction to their respective topics, lists of the
necessary material and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough
and cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocols
emphasizes the diversity of the research taking place in bacterial cell wall
homeostasis, and explains how the integration of information from across
multiple disciplines is going to be essential if a holistic understanding of
this important process is to be obtained.
Atomic
Force Microscopic Analysis of Bacterial Cell Wall Peptidoglycan Architecture.- Ultra-Sensitive
High Throughput Analytical Methods to Identify Novel Components Involved in Cell
Wall Biology and to Study Bacterial Cell Wall Dynamics.- Microarray
Analysis to Monitor Bacterial Cell Wall Homeostasis.- Cell Shaving
and False Positive Control Strategies Coupled to Novel Statistical Tools to Profile
Gram-Positive Bacterial Surface Proteomes.- Differential
Proteomics Based on Multidimensional Protein Identification Technology to Understand
the Biogenesis of Outer Membrane of Escherichia
coli.- Random
Transposon Mutagenesis for Cell-Envelope Resistant to Phage Infection.- Zymographic
Techniques for the Analysis of Bacterial Cell Wall in Bactillus.- Liquid Chromatography-Tandem Mass Spectrometry
to Define Sortase Cleavage Products.- Genetics
and Cell Morphology Analyses of the Actinomyces
oris srtA Mutant.- Construction
of a Bioassay System to Identify Extracellular Agents Targeting Bacterial Cell Envelope.- Luciferase
Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus.- Extraction
and Analysis of Peptidoglycan Cell Wall Precursors.- Continuous
Fluorescence Assay for Peptidoglycan Glycosyltransferases.- Analysis
of Peptidoglycan Fragment Release.- Analysis of Cell Wall Teichoic Acids in Staphylococcus aureus.- Analysis of Bacterial Cell Surface
Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.- Biophysical Measurements of Bacterial
Cell Shape.- Coarse-Grained Molecular Dynamics Simulations
of the Bacterial Cell Wall.- Structural
Comparison and Simulation of Pneumococcal Peptidoglycan Hydrolase LytB.
| Erscheinungsdatum | 03.08.2018 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1440 |
| Zusatzinfo | 35 Illustrations, color; 26 Illustrations, black and white; XII, 288 p. 61 illus., 35 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | gene expression • high-throughput approaches • non-protein components of the cell wall • Peptidoglycan • single celled organism • subcellular structure |
| ISBN-10 | 1-4939-8114-5 / 1493981145 |
| ISBN-13 | 978-1-4939-8114-4 / 9781493981144 |
| Zustand | Neuware |
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