Brain Tumor Stem Cells

Methods and Protocols

Sheila K. Singh, Chitra Venugopal (Herausgeber)

Buch | Hardcover

254 Seiten

2018 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-8804-4 (ISBN)

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This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocolsserves as an ideal guide for researchers seeking to better understand the complexities of brain cancer.

Introduction to Brain Tumor Stem Cells.- Isolation and Culture of Glioblastoma Brain Tumor Stem Cells.- Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines.- Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors.- Detecting Stem Cell Marker Expression Using the NanoString nCounter System.- Flow Cytometric Analysis of Brain Tumor Stem Cells.- In Vitro Self-Renewal Assays for Brain Tumor Stem Cells.- Differentiation of Brain Tumor Initiating Cells.- The Study of Brain Tumor Stem Cell Migration.- The Study of Brain Tumor Stem Cell Invasion.- Cell Cycle Dynamics in Glioma Cancer Stem Cells.- Embryonic Stem Cell Models of Human Brain Tumors.- Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory.- EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF.- Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells.- In Vitro Assays for Screening Small Molecules.- Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma.- Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci.- In Vivo Murine Models of Brain Metastasis.- Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain.

Erscheinungsdatum	26.10.2018
Reihe/Serie	Methods in Molecular Biology ; 1869
Zusatzinfo	34 Illustrations, color; 6 Illustrations, black and white; XII, 254 p. 40 illus., 34 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Neurologie
	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	Brain cancer • BTSC research • glioblastoma • Human cancer stem cell (CSC) populations • therapeutic options
ISBN-10	1-4939-8804-2 / 1493988042
ISBN-13	978-1-4939-8804-4 / 9781493988044
Zustand	Neuware