Insect Genomics

Methods and Protocols

Susan J. Brown, Michael E. Pfrender (Herausgeber)

Buch | Hardcover

237 Seiten

2018 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-8774-0 (ISBN)

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This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book provides an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover a range of useful topics such as determining genome size by flow cytometry; High Molecular Weight DNA extraction; improvements to a genome assembly provided by long-range sequencing approaches; assessments of orthology and single-copy genes at different phylogenetic levels; detecting regulatory regions with FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; bioinformatics analysis of epigenetic modifications, high-throughput scanning of insect genomes (TEEseq) for the presence of endosymbionts, and leveraging genome sequence information to design RNAi strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Insect Genomics: Methods and Protocols is a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field.

Arthropod Genome Sequencing and Assembly Strategies.- Genome Size Estimation and Quantitative Cytogenetics in Insects.- Isolation of HMW DNA from Insects Chapter.- Long range sequencing and assembly QC.- Integrated modeling of structural genes using MCuNovo.- Using BUSCO to Assess Insect Genomic Resources.- The GFF3toolkit—QC and Merge Pipeline for Genome Annotation.- Using Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) to identify functional regulatory DNA in insect genomes.- Using RAMPAGE to identify and annotate promoters in insect genomes.- CRM Beyond Model Insects.- Whole-genome bisulfite sequencing for the methylation analysis of insect genomes.- Bioinformatic analysis of methylation patterns using bisulfite sequencing data.- Physical Genome Mapping Using Fluorescence in Situ Hybridization with Mosquito Chromosomes.- Target enriched RAD sequencing (TEEseq): A new high-throughput sequencing approach applied to the comprehensive characterization of endosymbionts.

Erscheinungsdatum	12.11.2018
Reihe/Serie	Methods in Molecular Biology ; 1858
Zusatzinfo	23 Illustrations, color; 11 Illustrations, black and white; XI, 237 p. 34 illus., 23 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	arthropods • bioinformatics tools • Computational approaches • Developmental biology research • Genome sequencing • Toxicology research
ISBN-10	1-4939-8774-7 / 1493987747
ISBN-13	978-1-4939-8774-0 / 9781493987740
Zustand	Neuware