Autophagy in Differentiation and Tissue Maintenance

Methods and Protocols

Buch | Hardcover

228 Seiten

2018 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-8747-4 (ISBN)

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This detailed book compiles state-of-the-art protocols from researchers actively working in the area of autophagy, a crucial cellular process that regulates numerous cellular functions. In order to accelerate advances in the field, the volume explores aspects of autophagy research where a better understanding of its role is vitally important, such as in the maintenance of stem cell subpopulations and the regulation of differentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Autophagy in Differentiation and Tissue Maintenance: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of this key cell biological action.

Visualization and Measurement of Multiple Components of the Autophagy Flux.- Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells.- Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells.- Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement.- Autophagy in Adipocyte Differentiation.- Assessing Autophagy in the Leydig Cells.- Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells.- Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-Small Cell Lung Cancer Cells.- Autophagy in Zebrafish Extraocular Muscle Regeneration.- The Detection Techniques for Autophagy-Associated Cell Death Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry.- Cloning of Autophagy-Related MicroRNAs.- Assays to Monitor Aggrephagy in Drosophila Brain.- Methods for Monitoring Autophagy in Silkworm Organs.- Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia.- Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening.- Porcine Cell-Free System to Study Mammalian Sperm Mitophagy.- Monitoring and Measuring Mammalian Autophagy.

Erscheinungsdatum	18.09.2018
Reihe/Serie	Methods in Molecular Biology ; 1854
Zusatzinfo	38 Illustrations, color; 2 Illustrations, black and white; XII, 228 p. 40 illus., 38 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	cellular differentiation • Dysfunction and disease • Model organisms • Stem Cells • Stem cell subpopulations
ISBN-10	1-4939-8747-X / 149398747X
ISBN-13	978-1-4939-8747-4 / 9781493987474
Zustand	Neuware