Plant Pathogenic Fungi and Oomycetes
Humana Press Inc. (Verlag)
978-1-4939-8723-8 (ISBN)
Authoritative and cutting-edge, Plant Pathogenic Fungi and Oomycetes: Methods and Protocols aims to provide techniques developed through awide range of perspectives to facilitate research on a comprehensive understanding of these important filamentous pathogens.
Quantifying Re-association of a Facultative Endohyphal Bacterium with a Filamentous Fungus.- Characterizing Mycoviruses.- Analysis of Secondary Metabolites from Plant Endophytic Fungi.- Protocols for Investigating the Leaf Mycobiome using High throughput DNA Sequencing.- Characterizing Small RNAs in Filamentous Fungi using the Rice Blast Fungus, Magnaporthe oryzae, as an Example.- Plant Small RNAs Responsive to Fungal Pathogen Infection.- Sequential Phosphopeptide Enrichment for phosphoproteome Analysis of Filamentous Fungi: A Test Case using Magnaporthe oryzae.- Assays for MAP kinase Activation in Magnaporthe oryzae and other Plant Pathogenic Fungi.- Visualizing the Movement of Magnaporthe oryzae Effector Proteins in Rice Cells during Infection.- Illuminating Phytophthora Biology with Fluorescent Protein Tags.- Methods for Transient Gene Expression in Wild Relatives of Potato.- Host-induced Gene Silencing (HIGS) for Elucidating Puccinia Gene Function in Wheat.- From Short Reads to Chromosome-scale Genome Assemblies.- BLASTmap: A Shiny-based Application to Visualise BLAST Results as Interactive Heat Maps and a Tool to Design Gene Specific Baits for Bespoke Target Enrichment Sequencing.- A Computational Protocol to Analyze Meta-transcriptomic Data Capturing Fungal-host Interactions.- Application of the Cre/lox System to Construct Auxotrophic Markers for Quantitative Genetic Analyses in Fusarium graminearum.- Protocol of Phytophthora capsici Transformation using the CRISPR-Cas9 System.- Generating Gene Silenced Mutants in Phytophthora sojae.
Erscheinungsdatum | 19.09.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1848 |
Zusatzinfo | 36 Illustrations, color; 14 Illustrations, black and white; XI, 292 p. 50 illus., 36 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
Schlagworte | Arabidopsis • fungal chromatin • Gene Silencing • Magnaporthe oryzae • Phytophthora |
ISBN-10 | 1-4939-8723-2 / 1493987232 |
ISBN-13 | 978-1-4939-8723-8 / 9781493987238 |
Zustand | Neuware |
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