Mouse Retinal Phenotyping

Buch | Hardcover

310 Seiten

2018
Humana Press Inc. (Verlag)
978-1-4939-7719-2 (ISBN)

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The aim of this volume is to provide a wide range of techniques and aspects of mouse retinal phenotyping and characterization. Chapters guide readers through morphological and functional properties of the mouse retina and techniques for the analysis of outer to mid-retina and mid-to inner retina.

Written in the highly successful »Methods in Molecular Biology« series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mouse Retinal Phenotyping: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Part I: Overview
1 Morphological Survey from Neurons to Circuits of the Mouse Retina
Yoshihiko Tsukamoto
2 Measuring Retinal Function in the Mouse
Jan Kremers and Naoyuki Tanimoto
3 Modeling Retinal Diseases Using Genetic Approaches in Mice
Akiko Maeda and Tadao Maeda

Part II: Outer to Mid-Retina
4 Cell Culture Analysis of the Phagocytosis of Photoreceptor Outer Segments by Primary Mouse RPE Cells
Roni A. Hazim and David S. Williams
5 Two-photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (FLIM) of Retinal Pigment Epithelium (RPE) of Mice In Vivo
Yoko Miura
6 RPE Visual Cycle and Biochemical Phenotypes of Mutant Mouse Models
Bhubanananda Sahu and Akiko Maeda
7 Use of Direct Current Electroretinography for Analysis of Retinal Pigment Epithelium Function in Mouse Models
Minzhong Yu and Neal S. Peachey
8 Disruption of Rhodopsin Dimerization in Mouse Rod Photoreceptors by Synthetic Peptides Targeting Dimer Interface
Sandeep Kumar, Alyssia Lambert, Jon Rainier, and Yingbin Fu
9 Experimental Approaches for Defining the Role of the Ca2+-Modulated ROS-GC System in Retinal Rods of the Mouse
Clint L. Makino, Teresa Duda, Alexandre Pertzev, and Rameshwar K. Sharma
10 Microglia Analysis in Retinal Degeneration Mouse Models
Katharina Dannhausen, Khalid Rashid, and Thomas Langmann
11 Determination Of Mitochondrial Oxygen Consumption in the Retina Ex-vivo: Applications for Retinal Disease
Yogita K. Adlakha and Anand Swaroop
12 Analysis of Feedback Signaling from Horizontal Cells to Photoreceptors in Mice
Arlene A. Hirano, Xue Liu, Nicholas C. Brecha, and Steven Barnes
13 Assessment of the Absolute Excitatory Level of the Retina by Flicker Erg
Naoyuki Tanimoto and Mathias W. Seeliger
14 Ex Vivo Functional Evaluation of Synaptic Transmission from Rods to Rod Bipolar Cells in Mice
Johan Pahlberg, Anurima Majumder, and Nikolai O. Artemyev
15 Functional And Morphological Analysis of Off Bipolar Cells
Chase B. Hellmer and Tomomi Ichinose

Part III: Mid- To Inner Retina
16 Immunohistochemical Phenotyping Of Mouse Amacrine Cell Subtypes
Denize Atan
17 Phenotyping of Gap-junctional Coupling in the Mouse Retina
Arndt Meyer, Shubhash C. Yadav, and Karin Dedek
18 Ganglion Cell Assessment in Rodents with Retinal Degeneration
Erica L. Fletcher, Ursula Greferath, Susmita Saha, Emily E. Anderson, and Kirstan A. Vessey
19 Morphological Identification of Melanopsin Expressing Retinal Ganglion Cell Subtypes in Mice
Seul Ki Lee and Tiffany M. Schmidt
20 Functional Assessment of Melanopsin-driven Light Responses in the Mouse: Multielectrode Array Recordings
Shi-Jun Weng, Jordan M. Renna, Wei-Yi Chen, and Xiong-Li Yang
21 In Vitro Assays for Mouse Muller Cell Phenotyping through Microrna Profiling in the Damaged Retina
Luis I. Reyes-Aguirre, Heberto Quintero, Brenda Estrada-Leyva, and Monica Lamas
22 Analysis Of Retinal Vascular Plexi and Interplexus Connections
Aaron B. Simmons and Peter G. Fuerst

Erscheinungsdatum	10.04.2018
Reihe/Serie	Methods in Molecular Biology ; 1753
Zusatzinfo	74 col. Ill.; 51 b/w Ill.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Gewicht	848 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin
	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	amacrine cells • horizontal cells • Photoreceptor • Phototransduction • RPE function
ISBN-10	1-4939-7719-9 / 1493977199
ISBN-13	978-1-4939-7719-2 / 9781493977192
Zustand	Neuware