TGF-β Signaling

Methods and Protocols

Xin-Hua Feng, Pinglong Xu, Xia Lin (Herausgeber)

Buch | Softcover

394 Seiten

2016 | Softcover reprint of the original 1st ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-4993-9 (ISBN)

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This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF-β from the basic principles to the practical applications. Chapters provide basic introduction of TGF-β signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-β signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Regulation of TGF-b Receptors.- Determining TGF-β Receptor Levels in the Cell Membrane.- Post-translational Modifications of TGF-b Receptors.- Production, Isolation，and Structural Analysis of Ligands and Receptors of the TGF-b Superfamily.- Phosphorylation of Smads by Intracellular Kinases.- Analysis of Smad Phosphatase Activity in vitro.- Three-dimensional Mammary Epithelial Cell Morphogenesis Model for Analysis of TGF-b Signaling.- TGF-β Signaling in Stem Cell Regulation.- Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor β.- In vitro Th differentiation protocol.- Interrogating TGF-b Function and Regulation in Endothelial Cells.- Isolation and Manipulation of Adipogenic Cells to Assess TGF-b Superfamily Functions.- Imaging TGFb Signaling in Mouse Models of Cancer Metastasis.- Generation and Characterization of Smad7 Conditional Knockout Mice.- Monitoring Smad Activity in vivo Using the Xenopus Model System.- Animal Cap Assay for TGF-b Signaling.- Detection of Smad Signaling in Zebrafish Embryos.- Role of TGF-β signaling in Coupling Bone Remodeling.- Studying the Functions of TGF-β Signaling in the Ovary.- Quantitative Real-Time PCR Analysis of MicroRNAs and Their Precursors Regulated byTGF-b Signaling.- TGF-β Regulated microRNAs and Their Function in Cancer Biology.- Epigenomic Regulation Of Smad1 Signaling During Cellular Senescence Induced by Ras Activation.- The Role of Ubiquitination to Determine Non-Smad Signaling Responses.- Genome-wide RNAi Screening to Dissect the TGF-b Signal Transduction Pathway.- Measuring TGF-β Ligand Dynamics in Culture Medium.

Erscheinungsdatum	19.08.2017
Reihe/Serie	Methods in Molecular Biology ; 1344
Zusatzinfo	XII, 394 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	adipogenesis • bone remodeling • cell surface • Epigenetic regulation • epithelial-mesenchymal transition • intracellular kinases • microRNA • non-Smad pathway • post-translational regulations • Th differentiation
ISBN-10	1-4939-4993-4 / 1493949934
ISBN-13	978-1-4939-4993-9 / 9781493949939
Zustand	Neuware