Protein Expression in Mammalian Cells
Humana Press Inc. (Verlag)
978-1-4939-6160-3 (ISBN)
Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.
Why Proteins in Mammalian Cells?.- Large Scale Transfection of Mammalian Cells.- Selection of High Expressing Mammalian Cells by Surface Display of Reporters.- Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles.- Transfection of Difficult-to-Transfect Primary Mammalian Cells.- Stable Protein Expression in Mammalian Cells Using Baculoviruses.- Using Matrix Attachment Regions to Improve Recombinant Protein Production.- Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells.- Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells.- Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells.- Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker.- Tools for Co-Producing Multiple Proteins in Mammalian Cells.- Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases.- Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA.- Methods for Constructing Clones for Protein Expression in Mammalian Cells.- Optimizing Transient Recombinant Protein Expression in Mammalian Cells.
Erscheinungsdatum | 19.08.2017 |
---|---|
Reihe/Serie | Methods in Molecular Biology ; 801 |
Zusatzinfo | XI, 276 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-4939-6160-8 / 1493961608 |
ISBN-13 | 978-1-4939-6160-3 / 9781493961603 |
Zustand | Neuware |
Haben Sie eine Frage zum Produkt? |
aus dem Bereich