Protein Chromatography
Humana Press Inc. (Verlag)
978-1-4939-6129-0 (ISBN)
Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.
A Digest of Protein Purification.- Gel-Filtration Chromatography.- Immunoaffinity Chromatography.- Avoiding Proteolysis During Protein Chromatography.- Scale-Up of Protein Purification: Downstream Processing Issues.- Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources.- Engineering Protein Stability.- Microfluidics in Protein Chromatography.- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.- Storage and Lyophilization of Pure Proteins.- Differential Precipitation and Solubilization of Proteins.- Ion-Exchange Chromatography: Basic Principles and Application to the Partial Purification of Soluble Mammalian Prolyl Oligopeptidase.- Protein Quantitation and Analysis of Purity.- Purification of Proteins Fused to Glutathione S-Tranferase.- Purification of Proteins Fused to Maltose-Binding Protein.- Purification of Proteins from Baculovirus-Infected Insect Cells.- Purification of Poly-Histidine-Tagged Proteins.- Immobilized Metal Affinity Chromatography/Reversed-Phase Enrichment of Phosphopeptides and Analysis by CID/ETD Tandem Mass Spectrometry.- Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins.- Purification of Antibodies Using Affinity Chromatography.- Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens.- Measuring Protein-Protein Interactions Using Biacore.- Ultra-Performance Liquid Chromatography-Mass Spectrometry of Proteins.- Hydrophobic Interaction Chromatography.- Fast Protein Liquid Chromatography.- Shotgun Proteomics: A Qualitative Approach Applying Isoelectric Focusing on Immobilized pH Gradient and LC-MS/MS.- Shotgun Proteomics: A Relative Quantitative Approach Using Off-Gel Electrophoresis and LC-MS/MS.- Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems.- Strategies for the Purification of Membrane Proteins.- A Multi-Step ChromatographicStrategy to Purify Three Fungal Endo-β-Glucanases.
Erscheinungsdatum | 19.08.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 681 |
Zusatzinfo | X, 527 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Chemie ► Analytische Chemie | |
ISBN-10 | 1-4939-6129-2 / 1493961292 |
ISBN-13 | 978-1-4939-6129-0 / 9781493961290 |
Zustand | Neuware |
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