Antiviral RNAi
Humana Press Inc. (Verlag)
978-1-4939-5825-2 (ISBN)
Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.
Defense and counter-defense in the RNAi-based antiviral immune system in insects.- RNAi and cellular miRNAs in infections by mammalian viruses.- Viral miRNAs.- Progress in RNAi-based antiviral therapeutics.- Chemical modification of small interfering RNA.- Viral small RNA cloning and sequencing.- Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets.- Computational prediction of viral miRNAs.- Detection of Viral microRNAs by Northern Blot Analysis.- Detection of viral microRNA with S1 Nuclease Protection assay.- Characterization of RISC associated adenoviral small RNAs.- Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture.- Dicer assay in Drosophila S2 cell extract.- Slicer activity in Drosophila melanogaster S2 extract.- Gel Mobility Shift Assays for RNA-binding viral RNAi suppressors.- dsRNA uptake in adult Drosophila.- Design of small interfering RNAs for antiviral applications.- RNAi inducing lentiviral vectors for anti-HIV-1 gene therapy.- Production of multicopy shRNA lentiviral vectors for antiviral therapy.- Intranasal delivery of antiviral siRNA.- Antibody mediated delivery of siRNAs for anti-HIV therapy.- Aptamer-targeted RNAi for HIV-1 therapy.- RNAi screening for host factors involved in viral infection using Drosophila cells.- Genome-wide RNAi Screen for ViralReplication in Mammalian Cell Culture.- RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infections.
Erscheinungsdatum | 19.08.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 721 |
Zusatzinfo | 18 Illustrations, color; 21 Illustrations, black and white; XI, 425 p. 39 illus., 18 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-4939-5825-9 / 1493958259 |
ISBN-13 | 978-1-4939-5825-2 / 9781493958252 |
Zustand | Neuware |
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