Mutational adaptation of E. coli cells with novel molecular biosensors

Analysis of the mutational adaptation of E. coli to produce NADPH and malonyl-CoA with novel molecular biosensors and cultivation systems

Natascha Kowarsch (Autor)

Buch | Softcover

100 Seiten

2017
AV Akademikerverlag
978-3-330-52103-2 (ISBN)

Titel nicht im Sortiment

Artikel merken

Fatty acids play an important role in production of biodiesel as well as cosmetics and health products. One of the limiting factors in the fatty acid biosynthesis is NADPH, which is required for the reduction steps in the FAB cycle. An option to overcome this problem is the engineering of NADPH overproducing E. coli strains. Siedler et al. developed a biosensor based on the transcriptional regulator SoxR for NADPH-consuming enzymes. The activation of SoxR by high NADPH levels leads to the transcription of the SoxRS regulon. This regulon can contain many different genes and therefore can be designed to select for NADPH overproducing strains. In order to achieve this, the long-term mutational adaption of the engineered E. coli cells was tested in an ALE experiment. Through Next Generation sequencing of the genomes, a mutation in the ygcW gene was found, which could lead to a higher NADPH production.

Kowarsch, Natascha
2011-2014: FH Technikum Wien - Biomedical Engineering with specialsation in Tissue Enigneering; 2014-2016: IMC Krems - medical and pharmaceutical Biotechnology; 2015-2016: Student researcher at DTU Biosustain; 2017- : Project Manager at ABF pharmaceutical services GmbH;

Erscheinungsdatum	14.08.2017
Sprache	englisch
Maße	150 x 220 mm
Gewicht	165 g
Themenwelt	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	ALE • Biosensor • mini-fermentation • SoxR
ISBN-10	3-330-52103-1 / 3330521031
ISBN-13	978-3-330-52103-2 / 9783330521032
Zustand	Neuware