Phage Display

Methods and Protocols

Michael Hust, Theam Soon Lim (Herausgeber)

Buch | Hardcover

562 Seiten

2017 | 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-7446-7 (ISBN)

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This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display.

This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.

Construction of Human Immune and Naïve scFv Libraries.- Construction of Naïve and Immune Human Fab Phage Display Library.- Construction of Synthetic Antibody Phage Display Libraries.- Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene Cassettes.- Construction of Macaque Immune Libraries.- Construction of Bovine Immunoglobulin Libraries in the single chain Fragment variable (scFv) Format.- Construction of Rabbit Immune Antibody Libraries.- Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody Libraries.- Construction of High-Quality Camel Immune Antibody Libraries.- Construction of Chicken Antibody Libraries.- Construction and Selection of Affilin Phage Display Libraries.- Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies.- Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach.- Parallelized Antibody Selection in Microtiter Plates.- Mass Spectrometry Immuno Assay (MSIA ™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning.- Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection.- Phage Display and Selections on Cells.- Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens.- Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain Antibodies.- Screening Phage Display Antibody Libraries using Protein Arrays.- Antibody Selection on FFPE Tissue Slides.- Antibody Affinity and Stability Maturation by Error Prone PCR.- Upgrading Affinity Screening Experiments by Analysis of Next Generation Sequencing Data.- Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Library Construction.- High-Throughput IgG Reformatting and Expressions.- Monitoring Phage Biopanning by Next-Generation Sequencing.- ORFeome Phage Display.- Epitope Mapping by Phage Display.- Metasecretome Phage Display.- Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages.

Erscheinungsdatum	30.11.2017
Reihe/Serie	Methods in Molecular Biology ; 1701
Zusatzinfo	43 Illustrations, color; 17 Illustrations, black and white; XIV, 562 p. 60 illus., 43 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Schlagworte	antibodies • Array • DARPINs • IgNAR • macaque immune libraries • scFv libraries
ISBN-10	1-4939-7446-7 / 1493974467
ISBN-13	978-1-4939-7446-7 / 9781493974467
Zustand	Neuware