Bacteriophages

Methods and Protocols, Volume 3

Martha R.J. Clokie, Andrew M. Kropinski, Rob Lavigne (Herausgeber)

Buch | Hardcover

316 Seiten

2017 | 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-7341-5 (ISBN)

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This book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume III is a valuable resource for both established and novice phage scientists.

Basic Phage Mathematics.- Analysis of Host-Takeover during SPOi Infection of Bacillus subtilis.- Practical Advice on the One-Step Growth Curve.- Iron Chloride Flocculation of Bacteriophages from Seawater.- Purification of Bacteriophages using Anion-Exchange Chromatography.- Encapsulation Strategies of Bacteriophages (Felix O1) for Oral Therapeutic Application.- Encapsulation of Listeria Phage A511 by Alginate to Improve its Thermal Stability.- Application of a Virucidal Agent to Avoid Over-Estimation of Phage Kill during Phage Decontamination Assays on Ready-to-Eat Meats.- Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes.- Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology.- Analyzing Genome Termini of Bacteriophage Through High-Throughput Sequencing.- Amplification for Whole Genome Sequencing of Bacteriophages from Single Isolated Plaques using SISPA.- Genome Sequencing of dsDNA-Containing Bacteriophages Directly from a Single Plaque.- Preparing cDNA Libraries from Lytic Phage Infected Cells for Whole Transcriptome Analysis by RNA-Seq.- Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis.- Annotation of Bacteriophage Genome Sequences using DNA Master: An Overview.- Phage Genome Annotation using the RAST Pipeline.- Visualization of Phage Genomic Data: Comparative Genomics and Publication-Quality Diagrams.- Transposable Bacteriophages as Genetic Tools.- Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes.- Use of RP4::mini-Mu for Gene Transfer.- Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu.

Erscheinungsdatum	08.12.2017
Reihe/Serie	Methods in Molecular Biology ; 1681
Zusatzinfo	36 Illustrations, color; 25 Illustrations, black and white; XI, 316 p. 61 illus., 36 illus. in color. With online files/update.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	177 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	bacterial virus research • CRISPR/cas genome editing • gene predictions • genome sequences • High-throughput sequencing • RNAseq • Synthetic biology • systematic transcriptome analysis • systematic transcriptome analysis using RNAseq
ISBN-10	1-4939-7341-X / 149397341X
ISBN-13	978-1-4939-7341-5 / 9781493973415
Zustand	Neuware