Avian and Reptilian Developmental Biology

Methods and Protocols

Buch | Hardcover

365 Seiten

2017 | 1st ed. 2017
Humana Press Inc. (Verlag)
978-1-4939-7215-9 (ISBN)

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This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic manipulation; Part III focuses on methods to handle pluripotent cells; and part IV looks at the emerging models in avian and reptilian developmental biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Avian and Reptilian Developmental Biology: Methods and Protocols explores a var

iety of approaches and different sauropsid models that will help facilitate communication and collaboration among researchers, which in turn will progress this field forward.

Some Thoughts on Experimental Design.- Comparative Genomics as a Foundation for Evo-Devo Studies in Birds.- A Step-by-Step Guide to Assemble a Reptilian Genome.- Genomic and Transcriptomic Analyses of Avian Sex Chromosomes and Sex-Linked Genes.- Systems Biology Analyses in Chicken: Workflow for Transcriptome and ChIP-Seq Analyses using the Chicken Skin Paradigm.- Application of a CAGE Method to an Avian Development Study.- CRISPR/Cas9 in the Chicken Embryo.- Fluorescent Quail: A Transgenic Model System for the Dynamic Study of Avian Development.- Lentiviral-Mediated Transgenesis in Songbirds.- In Ovo Electroporation Methods in Chick Embryos.- Genetic Manipulation of the Avian Urogenital System using In Ovo Electroporation.- Enhancer Analyses using Chicken Embryo Electroporation.- Transgene Introduction into the Chick Limb Bud by Electroporation.- Chicken Induced Pluripotent Stem Cells: Establishment

and Characterization.- Isolation and Characterization of Chicken Primordial Germ Cells and their Application in Transgenesis.- Handling of Gametes for In Vitro Insemination in Birds.- In Vitro and Ex Ovo Culture of Reptilian and Avian Neural Progenitor Cells.- Lifting the Veil on Reptile Embryology: The Veiled Chameleon (Chamaeleo calyptratus) as a Model System to Study Reptilian Development.- Model Clades versus Model Species: Anolis Lizards as an Integrative Model of Anatomical Evolution.- The Feather Model for Chemo- and Radiation Therapy-Induced Tissue Damage.- An Early Chick Embryo Culture Device for Extended Continuous Observation.- A Sensitive and Versatile In Situ Hybridization Protocol for Gene Expression Analysis in Developing Amniote Brains.- Somitogenesis and Axial Development in Reptiles.- Micro-CT Imaging on Living Alligator Teeth Reveals Natural Tooth Cycling.

Erscheinungsdatum	06.09.2017
Reihe/Serie	Methods in Molecular Biology ; 1650
Zusatzinfo	75 Illustrations, color; 16 Illustrations, black and white; XII, 365 p. 91 illus., 75 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	genetic manipulation • gonad differentiation • Paleo • Segmentation • Stem Cell • Transcriptomics • Turtle model
ISBN-10	1-4939-7215-4 / 1493972154
ISBN-13	978-1-4939-7215-9 / 9781493972159
Zustand	Neuware