Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis

Bin Zhuang (Autor)

Buch | Hardcover

114 Seiten

2017 | 1st ed. 2018
Springer Verlag, Singapore
978-981-10-4752-7 (ISBN)

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This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successfuldevelopment and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.

For internal review: Dr. Bin Zhuang Supervisor: Prof. Jing Cheng, School of Medicine, Tsinghua University Current Affiliation: National Engineering Research Center for Beijing Biochip Technology, Capitalbio Corporation Research Area: Biomedical engineering, microfluidic, nucleotide analysis, electrophoresis, biochemistry Publications: [1] Gan, W.P.,# Zhuang, B.,# Zhang, P.F., Han, J.P., Li, C.X., and Liu, P. (2014). A filter paper-based microdevice for low-cost, rapid, and automated DNA extraction and amplification from diverse sample types. Lab on a Chip 14, 3719-3728. [2] Zhuang, B., # Gan, W.P., Wang, # S.Q., Han, J.P., Xiang, G.X., Li, C.X., Sun, J., Liu, P. (2015). A fully automated sample preparation microsystem for genetic testing of hereditary hearing loss using two-color multiplex allele-specific PCR. Analytical Chemistry, 87(2):1202-1209. [3] Zhuang, B., # Han, J.P., # Xiang, G.X., Gan, W.P., Wang, S.Q., Wang, D., Wang, L., Sun, J., Li, C.X., Liu, P. (2016). A fully integrated and automated microsystem for rapid pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. Lab on a Chip 16, 86-95.

Introduction.- The construction of a general platform for capillary electrophoresis.- Integrated module for automatic DNA extraction and amplification.- A fully-integrated genetic analysis system.- Conclusion and prospects for future work.

Erscheinungsdatum	05.08.2017
Reihe/Serie	Springer Theses
Zusatzinfo	44 Illustrations, color; 14 Illustrations, black and white; XXIII, 114 p. 58 illus., 44 illus. in color.
Verlagsort	Singapore
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Medizin / Pharmazie ► Physiotherapie / Ergotherapie ► Orthopädie
	Naturwissenschaften ► Biologie ► Biochemie
	Technik ► Maschinenbau
	Technik ► Medizintechnik
	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	Automatic Genetic Analysis • Capillary electrophoresis • Composite Chip • Congenital Hearing Loss • DNA Extraction System • Integrated Module • Micro-fluidic Technology • PCR Microchip
ISBN-10	981-10-4752-9 / 9811047529
ISBN-13	978-981-10-4752-7 / 9789811047527
Zustand	Neuware