A Structure-Function Toolbox for Membrane Transporter and Channels

Buch | Hardcover

302 Seiten

2017
Academic Press Inc (Verlag)
978-0-12-812353-9 (ISBN)

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A Structure-Function Toolbox for Membrane Transporter and Channels, Volume 594, a new release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters in the updated serial include Cryo-EM on membrane proteins embedded in nanodics, Solid-Supported membrane-based electrophysiology on membrane transporters and channels, Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, Single-molecule FRET on transporters, Dynamics of channels and transporters investigated by NMR, Structure-function studies on channels and transporters, and a section on MemStar, a new GFP-based expression and purification system for transporters and channels.

Prof. Dr. Christine Ziegler works at the Department of Structural Biology, Max Planck Institute of Biophysics, Germany

1. Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM 2. SSM-Based Electrophysiology for Transporter Research 3. Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters 4. Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy 5. The MEMbrane Protein Single ShoT Amplification Recipe: MemStar 6. Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli 7. Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling 8. Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR 9. Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein

Erscheinungsdatum	18.08.2017
Reihe/Serie	Methods in Enzymology
Verlagsort	San Diego
Sprache	englisch
Maße	152 x 229 mm
Gewicht	610 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
ISBN-10	0-12-812353-2 / 0128123532
ISBN-13	978-0-12-812353-9 / 9780128123539
Zustand	Neuware