Studies of Pluripotency in Embryonic Stem Cells and Induced Pluripotent Stem Cells

Xiaoyang Zhao (Autor)

Buch | Softcover

92 Seiten

2016 | Softcover reprint of the original 1st ed. 2014
Springer (Verlag)
978-94-024-0226-1 (ISBN)

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Stem cells have the ability to differentiate into all types of cells within the body, thus have great therapeutic potential for regenerative medicine to treat complicated disorders, like Parkinson’s disease and spinal cord injury. There will also be many applications in drug development. However, several roadblocks, such as safety issues and low efficiency of pluripotent stem cell (PSC) line derivation need to be resolved before their clinical application. This thesis focuses on these two areas, so as to find methods to overcome the limitation. It covers deriving embryonic stem cells (ESCs) from several different species and reports an efficient system to generate induced pluripotent stem cells (iPSCs), and the first iPSC mice in the world. The results in this thesis confirm that somatic cells can be fully reprogrammed with the four Yamanaka factors. In addition, we have found that the Dlk1-Dio3 region can be a potential molecular marker to distinguish the fully reprogrammed iPSCs from partially reprogrammed ones. All of these results will help improve the safety of PSCs in the clinical applications and increase the current low induction efficiency of their production.

Xiaoyang Zhao obtained his Ph.D. from the Institute of Zoology, Chinese Academy of Science (CAS). He was supervised by Professor Qi Zhou, who is one of the most famous researchers in stem cells and regenerative medicine. During his Ph.D. study, Xiaoyang Zhao published 9 research articles as the first author in well-known international journals, such as Nature, Cell Research and the Journal of Biological Chemistry. He received the Excellent Graduates award from the Institute of Zoology, CAS and the first ISTT Young Investigator Award from the International Society for Transgenic Technologies (ISTT) in 2011. Dr. Xiaoyang Zhao is currently a principle investigator at the Institute of Zoology, CAS.

Abstract.- Introduction.- Establishment of highly efficient somatic cell reprogramming system to generate iPSC lines- Establishment of highly efficient somatic cell reprogramming system and establishment of iPSC lines.- Pluripotency of iPSC and underlining mechanism.- Developmental potential of mouse iPSC.- Conclusions.

Erscheinungsdatum	28.08.2016
Reihe/Serie	Springer Theses
Zusatzinfo	18 Illustrations, color; 3 Illustrations, black and white; XV, 92 p. 21 illus., 18 illus. in color.
Verlagsort	Dordrecht
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	94-024-0226-8 / 9402402268
ISBN-13	978-94-024-0226-1 / 9789402402261
Zustand	Neuware